Literature summary for 4.2.3.121 extracted from

Landmann, C.; Fink, B.; Festner, M.; Dregus, M.; Engel, K.H.; Schwab, W.
Cloning and functional characterization of three terpene synthases from lavender (Lavandula angustifolia) (2007), Arch. Biochem. Biophys., 465, 417-429.

Search for more literature for 4.2.3.121

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Lavandula angustifolia

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0474	-	geranyl diphosphate	pH 7.5, 23°C	Lavandula angustifolia

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	or Mn2+, required, optimum concentration 50 mM	Lavandula angustifolia
Mn2+	or Mg2+, required, optimum concentration 0.2 mM. 68% of the efficiency with Mg2+, with change in product pattern to 46% limonene, 9% terpinolene, 23% alpha-pinene, 5% beta-myrcene and 4% alpha-phellandrene, for Mn2+	Lavandula angustifolia

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
70300	-	x * 70300, calculated	Lavandula angustifolia

Organism

Organism	UniProt	Comment	Textmining
Lavandula angustifolia	Q2XSC6	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
geranyl diphosphate	-	Lavandula angustifolia	(+)-alpha-pinene + diphosphate	products are 39% (R)-(+)-limonene, 22% terpinolene, 16% (1R,5S)-(+)-camphene, 14% (1R,5R)-(+)-alpha-pinene, 8% beta-myrcene and traces of alpha-phellandrene. Preferential formation of the (+)-enantiomers with 94% enantiomeric purity for (1R,5R)-(+)-alpha-pinene. Product pattern changes to 46% limonene, 9% terpinolene, 23% alpha-pinene, 5% beta-myrcene and 4% alpha-phellandrene, when Mn2+ is supplied instead of Mg2+	?

Subunits

Subunits	Comment	Organism
?	x * 70300, calculated	Lavandula angustifolia

Synonyms

Synonyms	Comment	Organism
limonene synthase	-	Lavandula angustifolia
LIMS	-	Lavandula angustifolia

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	optimum for total enzymic activity	Lavandula angustifolia
33	-	optimum for maximum formation of alpha-pinene	Lavandula angustifolia

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.012	-	geranyl diphosphate	pH 7.5, 23°C	Lavandula angustifolia

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	optimum for maximum formation of alpha-pinene	Lavandula angustifolia
7	-	optimum for total enzymic activity	Lavandula angustifolia

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.253	-	geranyl diphosphate	pH 7.5, 23°C	Lavandula angustifolia

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)