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Literature summary for 4.2.3.12 extracted from
- Purification and characterization of 6-pyruvoyl tetrahydropterin synthase from salmon liver (1989), Eur. J. Biochem., 180, 205-211.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithioerythritol | increased catalytic activity with 10 mM | Salmo salar |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| N-(7-Dimethyl-amino-4-methyl coumarinyl) maleimide | - |
Salmo salar | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0022 | - |
6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin | - |
Salmo salar | |
| 0.0022 | - |
7,8-dihydroneopterin triphosphate | pH 7.4, 37ºC | Salmo salar | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 16000 | - |
x * 16000, SDS-PAGE | Salmo salar |
| 17000 | - |
x * 17000, SDS-PAGE | Salmo salar |
| 68000 | - |
gel filtration | Salmo salar |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin | Salmo salar | one of the enzymes involved in tetrahydrobiopterin biosynthesis | 6-(1,2-dioxopropyl)-5,6,7,8-tetrahydropterin + triphosphate | - |
? | |
| 7,8-dihydroneopterin triphosphate | Salmo salar | assay at | 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salmo salar | - |
salmon | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| additional information | free of mannose and glucose residues | Salmo salar |
| no glycoprotein | no carbohydrates bound | Salmo salar |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, hydrophobic interaction chromatography, gel filtration, ion exchange, hydroxyapatite chromatography | Salmo salar |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Salmo salar | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.6 | - |
- |
Salmo salar |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin | - |
Salmo salar | 6-(1,2-dioxopropyl)-5,6,7,8-tetrahydropterin + triphosphate | - |
? | |
| 6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin | one of the enzymes involved in tetrahydrobiopterin biosynthesis | Salmo salar | 6-(1,2-dioxopropyl)-5,6,7,8-tetrahydropterin + triphosphate | - |
? | |
| 7,8-dihydroneopterin triphosphate | - |
Salmo salar | 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate | - |
? | |
| 7,8-dihydroneopterin triphosphate | assay at | Salmo salar | 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 16000, SDS-PAGE | Salmo salar |
| ? | x * 17000, SDS-PAGE | Salmo salar |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
5 min, 25% loss of activity | Salmo salar |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Salmo salar |
| 7.5 | - |
in Tris-HCl 100 mM | Salmo salar |
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