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Literature summary for 4.2.3.12 extracted from
- Purification and cDNA cloning of rat 6-pyruvoyl-tetrahydropterin synthase (1991), J. Biol. Chem., 266, 20791-20796.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithioerythritol | increased catalytic activity with 10 mM | Rattus norvegicus |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloned | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ammonium sulfate | 50% inactivation at 400 mM | Rattus norvegicus | |
| additional information | NaCl 100 mM and KCl 100 mM have no effect on activity | Rattus norvegicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0091 | - |
6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin | - |
Rattus norvegicus | |
| 0.0091 | - |
7,8-dihydroneopterin triphosphate | pH 7.4, 37ºC | Rattus norvegicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 65% of the activity with equal concentration of Mg2+ | Rattus norvegicus | |
| Mg2+ | optimum concentration is 8 mM | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 17000 | - |
x * 17000, SDS-PAGE | Rattus norvegicus |
| 17000 | - |
4 * 17000, SDS-PAGE | Rattus norvegicus |
| 83000 | - |
HPLC gel filtration | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7,8-dihydroneopterin triphosphate | Rattus norvegicus | assay at | 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | P27213 | Wistar, cloned | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| additional information | mature protein lacks 4 N-terminal amino acids residues | Rattus norvegicus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, hydroxyapatite column, butyl-toyopearl chromatography, gel filtration, HPLC ion exchange column | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.2883 | - |
- |
Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, stable for at least 1 year | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7,8-dihydroneopterin triphosphate | - |
Rattus norvegicus | 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate | - |
? | |
| 7,8-dihydroneopterin triphosphate | assay at | Rattus norvegicus | 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 17000, SDS-PAGE | Rattus norvegicus |
| tetramer | 4 * 17000, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7 | - |
Rattus norvegicus |
| 7.4 | - |
assay at | Rattus norvegicus |
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Release 2023.1 (January 2023)
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