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Literature summary for 4.2.3.118 extracted from

  • Wang, C.M.; Cane, D.E.
    Biochemistry and molecular genetics of the biosynthesis of the earthy odorant methylisoborneol in Streptomyces coelicolor (2008), J. Am. Chem. Soc., 130, 8908-8909.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.3.118

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptomyces coelicolor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0028
-
 geranyl diphosphate pH 7.0, 30°C Streptomyces coelicolor
0.026
-
 (E)-2-methylgeranyl diphosphate pH 7.0, 30°C Streptomyces coelicolor

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49937
-
 x * 49937, MALDI-TOF Streptomyces coelicolor

Organism

Organism UniProt Comment Textmining
Streptomyces coelicolor Q9F1Y6
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Streptomyces coelicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(E)-2-methylgeranyl diphosphate + H2O
-
 Streptomyces coelicolor 2-methylisoborneol + diphosphate plus trace amounts of 2-methylbornene ?
geranyl diphosphate + H2O
-
 Streptomyces coelicolor limonene + diphosphate products are 6% alpha-pinene, 23% beta-pinene , 32% limonene, 29% gamma-terpinene, and 120% delta-terpinene ?
additional information cyclization of (E)-2-methylgeranyl diphosphate most likely occurs by a mechanism closely resembling that established for the conversion of geranyl diphosphate to bornyl diphosphate, except that the penultimate bornyl cation is quenched on the exo face by water, analogous to the formation of fenchol, rather than by internal return of the diphosphate to the endo face Streptomyces coelicolor ?
-
 ?
additional information no substrate: farnesyl diphosphate, geranylgeranyl diphosphate Streptomyces coelicolor ?
-
 ?

Subunits

Subunits Comment Organism
? x * 49937, MALDI-TOF Streptomyces coelicolor

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0003
-
 geranyl diphosphate pH 7.0, 30°C Streptomyces coelicolor
0.039
-
 (E)-2-methylgeranyl diphosphate pH 7.0, 30°C Streptomyces coelicolor