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Literature summary for 4.2.3.115 extracted from

  • Jin, J.; Kim, M.J.; Dhandapani, S.; Tjhang, J.G.; Yin, J.L.; Wong, L.; Sarojam, R.; Chua, N.H.; Jang, I.C.
    The floral transcriptome of ylang ylang (Cananga odorata var. fruticosa) uncovers biosynthetic pathways for volatile organic compounds and a multifunctional and novel sesquiterpene synthase (2015), J. Exp. Bot., 66, 3959-3975 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.3.115

Cloned(Commentary)

Cloned (Comment) Organism
gene TPS1, sequence comparisons and phylogenetic analysis and tree, quantitative real-time PCR enzyme expression analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS, recombinant expression of YFP-tagged enzyme containing the N-terminal plastid Tp sequence in Nicotiana benthamiana leaf chloroplasts under control of CaMV 35S promoter via transformation method with Agrobacterium tumefaciens strain GV3101 Cananga odorata var. fruticosa

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Cananga odorata var. fruticosa 9507
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, enzyme CoTPS1 has the conserved aspartate-rich motif (DDXXD) and NSE/DTE motifs that chelate divalent metal ions, typically Mg2+, in the C-terminal domain Cananga odorata var. fruticosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
geranyl diphosphate Cananga odorata var. fruticosa
-
 alpha-terpinene + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Cananga odorata var. fruticosa
-
 dwarf ylang ylang, grown in Singapore
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography Cananga odorata var. fruticosa

Source Tissue

Source Tissue Comment Organism Textmining
flower mature yellow flower Cananga odorata var. fruticosa
-
additional information no alpha-terpinene production in flower bud, undeveloped small flowers, and mature green flowers Cananga odorata var. fruticosa
-
petal
-
 Cananga odorata var. fruticosa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geranyl diphosphate
-
 Cananga odorata var. fruticosa alpha-terpinene + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
CoTPS1
-
 Cananga odorata var. fruticosa
TPS1
-
 Cananga odorata var. fruticosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Cananga odorata var. fruticosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Cananga odorata var. fruticosa

General Information

General Information Comment Organism
evolution the enzyme belongs to the terpene sythase superfamily, subfamily TPS-b. The arginine-tryptophan motif, R(R)X8W, present at the N terminus of most mono-TPS and in some sesqui-TPS and di-TPS, is found in CoTPS1, and CoTPS1 contains a putative N-terminal plastid Tp sequence of 41 amino acids Cananga odorata var. fruticosa
metabolism the enzyme is part of the biosynthesis of volatile organic compounds in flowers Cananga odorata var. fruticosa
physiological function the enzyme is part of the biosynthesis of volatile organic compounds (VOCs) in flowers. Floral scents are a key factor in plant-insect interactions and are vital for successful pollination. The scented flowers of Cananga odorata var. fruticosa are analysed by GC-MS and a total of 49 VOCs are identified at four different stages of flower development. The bulk of these VOCs are terpenes, mainly sesquiterpenes. Enzyme CoTPS1 synthesizes four products corresponding to beta-thujene, sabinene, beta-pinene, and -alpha from geranyl diphosphate Cananga odorata var. fruticosa