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Literature summary for 4.2.3.114 extracted from
- gamma-Terpinene synthetase: A key enzyme in the biosynthesis of aromatic monoterpenes (1978), Arch. Biochem. Biophys., 191, 400-411.
General Stability
| General Stability | Organism |
|---|---|
| addition of either 20% glycerol or 1 mM substrate does not significantly improve stability | Thymus vulgaris |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 1 mM, complete inactivation | Thymus vulgaris |  |
| additional information | not inhibitory: gamma-terpinene, p-cymene, thymol | Thymus vulgaris |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.009 | - |
neryl diphosphate | pH 6.6, 30°C | Thymus vulgaris | |
| 0.014 | - |
geranyl diphosphate | pH 6.6, 30°C | Thymus vulgaris | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | or Mn2+, required, Mg2+ is preferred over Mn2+ | Thymus vulgaris | |
| Mn2+ | or Mg2+, required, about 60% of the efficiency qith Mg2+ | Thymus vulgaris | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 96000 | - |
gel filtration | Thymus vulgaris |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thymus vulgaris | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Thymus vulgaris |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| geranyl diphosphate = gamma-terpinene + diphosphate | mechanism involves the cyclizazion of the acyclic precursor, loss of a proton from C5 to form the delta4 double bond, and a 1,2-hydride shift fom C4 to C8 to give gamma-terpinene | Thymus vulgaris |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C, 40 mM sodium phosphate, 5 mM MgCl2, 0.5 mM dithioerythritol, loss of 60% of activity within 1 week | Thymus vulgaris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| geranyl diphosphate | - |
Thymus vulgaris | gamma-terpinene + diphosphate | - |
? | |
| neryl diphosphate | - |
Thymus vulgaris | gamma-terpinene + diphosphate | 75% gamma-terpinene, 20% alpha-thujene, 5% alpha-terpineol, partially purified enzyme | ? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
- |
Thymus vulgaris |
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Release 2023.1 (January 2023)
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