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Literature summary for 4.2.3.11 extracted from
- Monoterpene biosynthesis: demonstration of a geranyl pyrophosphate:sabinene hydrate cyclase in soluble enzyme preparations from sweet marjoram (Majorana hortensis) (1988), Arch. Biochem. Biophys., 264, 618-631.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| diphosphate | 0.1 mM: 30% stimulation | Origanum majorana |  |
General Stability
| General Stability | Organism |
|---|---|
| proteolytic degradation by 0.01 mg/ml trypsin, folowed by addition of soybean trypsin inhibitor: all cyclase activities are destroyed within 10 min, t1/2 is 3 min | Origanum majorana |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diphosphate | 0.5 mM: 80% inhibition | Origanum majorana | |
| EDTA | - |
Origanum majorana | |
| NaWO4 | 10 mM, 45% inhibition | Origanum majorana | |
| NEM | IC50: 0.08 mM | Origanum majorana | |
| NH4VO3 | 10 mM, 45% inhibition | Origanum majorana | |
| PCMB | IC50: 0.02 mM | Origanum majorana | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0019 | - |
geranyl diphosphate | pH 7.0, 30°C | Origanum majorana | |
| 0.0105 | - |
neryl diphosphate | pH 7.0, 30°C | Origanum majorana | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Origanum majorana | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | Mg2+ or Mn2+ required, Km: 2.4 mM | Origanum majorana | |
| Mn2+ | Mn2+ or Mg2+ required, Km: 0.3 mM | Origanum majorana | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56000 | - |
gel filtration | Origanum majorana |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Origanum majorana | - |
Moench | - |
| Origanum majorana Moench | - |
Moench | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Origanum majorana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| geranyl diphosphate | - |
Origanum majorana | sabinene hydrate + diphosphate | both cis- and trans-isomers of sabinene hydrate are formed | ? | |
| geranyl diphosphate | - |
Origanum majorana Moench | sabinene hydrate + diphosphate | both cis- and trans-isomers of sabinene hydrate are formed | ? | |
| neryl diphosphate | - |
Origanum majorana | sabinene hydrate + diphosphate | cis- and trans-sabinene hydrate | ? | |
| neryl diphosphate | - |
Origanum majorana Moench | sabinene hydrate + diphosphate | cis- and trans-sabinene hydrate | ? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
complete inactivation after 20 min | Origanum majorana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Origanum majorana |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.3 | 8 | 50% of maximal activity at pH 6.3 and 8.0 | Origanum majorana |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
IC50: 0.02 mM | Origanum majorana | PCMB | |
| 0.08 | - |
IC50: 0.08 mM | Origanum majorana | NEM | |
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