Activating Compound | Comment | Organism | Structure |
---|---|---|---|
diphosphate | 0.1 mM: 30% stimulation | Origanum majorana |
General Stability | Organism |
---|---|
proteolytic degradation by 0.01 mg/ml trypsin, folowed by addition of soybean trypsin inhibitor: all cyclase activities are destroyed within 10 min, t1/2 is 3 min | Origanum majorana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diphosphate | 0.5 mM: 80% inhibition | Origanum majorana | |
EDTA | - |
Origanum majorana | |
NaWO4 | 10 mM, 45% inhibition | Origanum majorana | |
NEM | IC50: 0.08 mM | Origanum majorana | |
NH4VO3 | 10 mM, 45% inhibition | Origanum majorana | |
PCMB | IC50: 0.02 mM | Origanum majorana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0019 | - |
geranyl diphosphate | pH 7.0, 30°C | Origanum majorana | |
0.0105 | - |
neryl diphosphate | pH 7.0, 30°C | Origanum majorana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Origanum majorana | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Mg2+ or Mn2+ required, Km: 2.4 mM | Origanum majorana | |
Mn2+ | Mn2+ or Mg2+ required, Km: 0.3 mM | Origanum majorana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
gel filtration | Origanum majorana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Origanum majorana | - |
Moench | - |
Origanum majorana Moench | - |
Moench | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Origanum majorana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranyl diphosphate | - |
Origanum majorana | sabinene hydrate + diphosphate | both cis- and trans-isomers of sabinene hydrate are formed | ? | |
geranyl diphosphate | - |
Origanum majorana Moench | sabinene hydrate + diphosphate | both cis- and trans-isomers of sabinene hydrate are formed | ? | |
neryl diphosphate | - |
Origanum majorana | sabinene hydrate + diphosphate | cis- and trans-sabinene hydrate | ? | |
neryl diphosphate | - |
Origanum majorana Moench | sabinene hydrate + diphosphate | cis- and trans-sabinene hydrate | ? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
complete inactivation after 20 min | Origanum majorana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Origanum majorana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.3 | 8 | 50% of maximal activity at pH 6.3 and 8.0 | Origanum majorana |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.02 | - |
IC50: 0.02 mM | Origanum majorana | PCMB | |
0.08 | - |
IC50: 0.08 mM | Origanum majorana | NEM |