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Literature summary for 4.2.3.109 extracted from

  • Roach, C.R.; Hall, D.E.; Zerbe, P.; Bohlmann, J.
    Plasticity and evolution of (+)-3-carene synthase and (-)-sabinene synthase functions of a Sitka spruce monoterpene synthase gene family associated with weevil resistance (2014), J. Biol. Chem., 289, 23859-23869.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.3.109

Cloned(Commentary)

Cloned (Comment) Organism
-
 Picea sitchensis

Protein Variants

Protein Variants Comment Organism
A595G shift in product profile, mutant produces 2.4% (+)-car-3-ene, 39% (-)-sabinene, 33.3% terpinolene Picea sitchensis
A595G/F596L/L599F shift in product profile, mutant produces 42.3% (+)-car-3-ene, 7.3% (-)-sabinene, 20.1% terpinolene Picea sitchensis
F596E shift in product profile, mutant produces 0.5% (+)-car-3-ene, 10.2% (-)-sabinene, 5.7% terpinolene, 70.9% limonene Picea sitchensis
F596G shift in product profile, mutant produces 10.0% (+)-car-3-ene, 11.2% (-)-sabinene, 17.3% terpinolene, 27.0% myrcene, 17.0% limonene Picea sitchensis
F596H shift in product profile, mutant produces 2.1% (+)-car-3-ene, 46.5% (-)-sabinene, 27.1% terpinolene Picea sitchensis
F596L shift in product profile, mutant produces 28.6% (+)-car-3-ene, 42.8% (-)-sabinene, 36.4% terpinolene Picea sitchensis
F596R inactive Picea sitchensis
L599F shift in product profile, mutant produces 1.5% (+)-car-3-ene, 20.2% (-)-sabinene, 32.2% terpinolene Picea sitchensis
additional information construction of a series of domain swaps and site-directed substitutions between (-)-sabinene synthase and (+)-3-carene synthase isoforms to explore which regions and specific amino acids of these enzymes affect the differences of their product profiles. The amino acid in position 596 is critical for product profiles dominated by or (-)-sabinene. A leucine in this position promotes formation of (+)-3-carene, whereas phenylalanine promotes (-)-sabinene Picea sitchensis
S589A shift in product profile, mutant produces 1.2% (+)-car-3-ene, 42.9% (-)-sabinene, 35.5% terpinolene Picea sitchensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0202
-
 geranyl diphosphate mutant A595G/F596L/L599F, 30°C, pH not specified in the publication Picea sitchensis
0.0316
-
 geranyl diphosphate mutant F596L, 30°C, pH not specified in the publication Picea sitchensis
0.0357
-
 geranyl diphosphate wild-type, 30°C, pH not specified in the publication Picea sitchensis

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Picea sitchensis 9507
-

Organism

Organism UniProt Comment Textmining
Picea sitchensis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geranyl diphosphate
-
 Picea sitchensis (-)-sabinene + diphosphate wild-type produces 1.3% (+)-car-3-ene, 44.7% (-)-sabinene, 35.9% terpinolene ?

Synonyms

Synonyms Comment Organism
TPS-sab
-
 Picea sitchensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0352
-
 geranyl diphosphate mutant A595G/F596L/L599F, 30°C, pH not specified in the publication Picea sitchensis
0.0825
-
 geranyl diphosphate mutant F596L, 30°C, pH not specified in the publication Picea sitchensis
0.545
-
 geranyl diphosphate wild-type, 30°C, pH not specified in the publication Picea sitchensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0017
-
 geranyl diphosphate mutant A595G/F596L/L599F, 30°C, pH not specified in the publication Picea sitchensis
0.0027
-
 geranyl diphosphate mutant F596L, 30°C, pH not specified in the publication Picea sitchensis
0.0153
-
 geranyl diphosphate wild-type, 30°C, pH not specified in the publication Picea sitchensis