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Literature summary for 4.2.3.108 extracted from

  • Ruan, J.X.; Li, J.X.; Fang, X.; Wang, L.J.; Hu, W.L.; Chen, X.Y.; Yang, C.Q.
    Isolation and characterization of three new monoterpene synthases from Artemisia annua (2016), Front. Plant Sci., 7, 638 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.3.108

Cloned(Commentary)

Cloned (Comment) Organism
cloning from a cDNA library, sequence comparisons and phylogenetic analysis and tree, quantitative RT-PCR expression analysis, recombinant expression of His-tagged enzyme without after removing the N-terminal plastid targeting sequence in Escherichia coli strain BL21(DE3) Artemisia annua

Protein Variants

Protein Variants Comment Organism
additional information the N-terminal signal peptide of AaTPS6 (46 amino acid residues) before RR motif is truncated and recombinantly expressed in Escherichia coli strain BL21(DE3) Artemisia annua

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics Artemisia annua
0.0177
-
 geranyl diphosphate pH 7.0, 30°C, recombinant enzyme Artemisia annua

Localization

Localization Comment Organism GeneOntology No. Textmining
amyloplast
-
 Artemisia annua 9501
-
chloroplast
-
 Artemisia annua 9507
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Artemisia annua
Mn2+ activates Artemisia annua
additional information the enzyme depends on the presence of divalent cations, Mg2+ is the preferred metal ion Artemisia annua

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
geranyl diphosphate + H2O Artemisia annua
-
 1,8-cineole + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Artemisia annua A0A068L889
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Artemisia annua

Source Tissue

Source Tissue Comment Organism Textmining
inflorescence
-
 Artemisia annua
-
leaf high enzyme expression level in young leaves Artemisia annua
-
additional information quantitative RT-PCR expression analysis in plants tissues, AaTPS6 is highly expressed in root and young leaves. All the monoterpene synthase genes show higher expression levels in young leaf than in mature leaf, consistent with the contents of monoterpenes Artemisia annua
-
root highest enzyme expression level Artemisia annua
-
stem
-
 Artemisia annua
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geranyl diphosphate + H2O
-
 Artemisia annua 1,8-cineole + diphosphate
-
 ?
additional information enzyme AaTPS6 produces multiple products with 1,8-cineole as major product (59.28% of total),in addition to ten other products including sabinene and beta-phellandrene (together 19.04%), alpha-terpineol (7.84%), trans-sabinene hydrate(4.03%), (-)-alpha-pinene (3.00%), cis-beta-terpineol (2.51%), beta-myrcene (2.01%), alpha-thujene (0.69%), as well as two unidentified minor products. No activity with farnesyl diphosphate or geranylgeranyl diphosphate Artemisia annua ?
-
 ?

Synonyms

Synonyms Comment Organism
AaTPS6
-
 Artemisia annua
TPS6
-
 Artemisia annua

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Artemisia annua

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 45 the recombinant enzyme AaTPS6 activity does not differ greatly at the range from 25°C to 40°C, it reaches peak at 25°C, has its maximal activity at 40°C, and retains 70% at 45°C, activity drops sharply above 40°C Artemisia annua

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.46
-
 geranyl diphosphate pH 7.0, 30°C, recombinant enzyme Artemisia annua

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Artemisia annua

Expression

Organism Comment Expression
Artemisia annua mechanical wounding induces the expression of all three monoterpene synthase genes, including TPS6, and transcript levels of AaTPS6 are also strongly elevated after treatments with phytohormones, induction by methyl jasmonate (8fold), salicylic acid (4fold), and gibberellin (12fold), suggesting a role of the monoterpene synthase in plant-environment interactions up

General Information

General Information Comment Organism
evolution enzyme AaTPS6 belongs to the terpene synthases family, TPS-b subfamily Artemisia annua
metabolism in Artemisia annua, three monoterpene synthases AaTPS2, AaTPS5, and AaTPS6, produce beta-myrcene, camphene, and 1,8-cineole as the major products, respectively Artemisia annua
physiological function the monoterpene synthase TPS6 might play a role in plant-environment interactions Artemisia annua

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
252
-
 geranyl diphosphate pH 7.0, 30°C, recombinant enzyme Artemisia annua