Cloned (Comment) | Organism |
---|---|
- |
Picea sitchensis |
Protein Variants | Comment | Organism |
---|---|---|
G595A/L596F/F599L | shift in product profile, mutant produces 1.8% (+)-car-3-ene, 23.3% (-)-sabinene, 55.2% terpinolene | Picea sitchensis |
G595A/L596F/F599L | shift in product profile, mutant produces 4.7% (+)-car-3-ene, 47.4% (-)-sabinene, 35.2% terpinolene | Picea sitchensis |
G595A/L596F/F599L | shift in product profile, mutant produces 5.4% (+)-car-3-ene, 29.0% (-)-sabinene, 26.4% terpinolene | Picea sitchensis |
L596F | shift in product profile, mutant produces 12.3% (+)-car-3-ene, 37.4% (-)-sabinene, 35.4% terpinolene | Picea sitchensis |
L596F | shift in product profile, mutant produces 5% (+)-car-3-ene, 20.9% (-)-sabinene, 53.5% terpinolene | Picea sitchensis |
L596F | shift in product profile, mutant produces 9.2% (+)-car-3-ene, 20.2% (-)-sabinene, 32.2% terpinolene | Picea sitchensis |
additional information | construction of a series of domain swaps and site-directed substitutions between (-)-sabinene synthase and (+)-3-carene synthase isoforms to explore which regions and specific amino acids of these enzymes affect the differences of their product profiles. The amino acid in position 596 is critical for product profiles dominated by (+)-3-carene in (+)-3-carene synthase isoforms. A leucine in this position promotes formation of (+)-3-carene, whereas phenylalanine promotes (-)-sabinene | Picea sitchensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0153 | - |
geranyl diphosphate | mutant L596F, 30°C, pH not specified in the publication | Picea sitchensis | |
0.0158 | - |
geranyl diphosphate | wild-type, 30°C, pH not specified in the publication | Picea sitchensis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Picea sitchensis | 9507 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Picea sitchensis | F1CKI6 | isoform TPS-3car1 | - |
Picea sitchensis | F1CKI8 | isoform TPS-3car2 | - |
Picea sitchensis | F1CKI9 | isoform TPS-3car3 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranyl diphosphate | - |
Picea sitchensis | (+)-car-3-ene + diphosphate | wild-type produces 46.2% (+)-car-3-ene, 8.8% (-)-sabinene, 29.7% terpinolene | ? | |
geranyl diphosphate | - |
Picea sitchensis | (+)-car-3-ene + diphosphate | wild-type produces 49% (+)-car-3-ene, 8.7% (-)-sabinene, 24.7% terpinolene | ? | |
geranyl diphosphate | - |
Picea sitchensis | (+)-car-3-ene + diphosphate | wild-type produces 67.5% (+)-car-3-ene, 6.9% (-)-sabinene, 15.4% terpinolene | ? |
Synonyms | Comment | Organism |
---|---|---|
(+)-3-carene synthase | - |
Picea sitchensis |
TPS-3car1 | - |
Picea sitchensis |
TPS-3car2 | - |
Picea sitchensis |
TPS-3car3 | - |
Picea sitchensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.098 | - |
geranyl diphosphate | wild-type, 30°C, pH not specified in the publication | Picea sitchensis | |
0.643 | - |
geranyl diphosphate | mutant L596F, 30°C, pH not specified in the publication | Picea sitchensis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0062 | - |
geranyl diphosphate | wild-type, 30°C, pH not specified in the publication | Picea sitchensis | |
0.042 | - |
geranyl diphosphate | mutant L596F, 30°C, pH not specified in the publication | Picea sitchensis |