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Literature summary for 4.2.3.107 extracted from

  • Roach, C.R.; Hall, D.E.; Zerbe, P.; Bohlmann, J.
    Plasticity and evolution of (+)-3-carene synthase and (-)-sabinene synthase functions of a Sitka spruce monoterpene synthase gene family associated with weevil resistance (2014), J. Biol. Chem., 289, 23859-23869.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.3.107

Cloned(Commentary)

Cloned (Comment) Organism
-
 Picea sitchensis

Protein Variants

Protein Variants Comment Organism
G595A/L596F/F599L shift in product profile, mutant produces 1.8% (+)-car-3-ene, 23.3% (-)-sabinene, 55.2% terpinolene Picea sitchensis
G595A/L596F/F599L shift in product profile, mutant produces 4.7% (+)-car-3-ene, 47.4% (-)-sabinene, 35.2% terpinolene Picea sitchensis
G595A/L596F/F599L shift in product profile, mutant produces 5.4% (+)-car-3-ene, 29.0% (-)-sabinene, 26.4% terpinolene Picea sitchensis
L596F shift in product profile, mutant produces 12.3% (+)-car-3-ene, 37.4% (-)-sabinene, 35.4% terpinolene Picea sitchensis
L596F shift in product profile, mutant produces 5% (+)-car-3-ene, 20.9% (-)-sabinene, 53.5% terpinolene Picea sitchensis
L596F shift in product profile, mutant produces 9.2% (+)-car-3-ene, 20.2% (-)-sabinene, 32.2% terpinolene Picea sitchensis
additional information construction of a series of domain swaps and site-directed substitutions between (-)-sabinene synthase and (+)-3-carene synthase isoforms to explore which regions and specific amino acids of these enzymes affect the differences of their product profiles. The amino acid in position 596 is critical for product profiles dominated by (+)-3-carene in (+)-3-carene synthase isoforms. A leucine in this position promotes formation of (+)-3-carene, whereas phenylalanine promotes (-)-sabinene Picea sitchensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0153
-
 geranyl diphosphate mutant L596F, 30°C, pH not specified in the publication Picea sitchensis
0.0158
-
 geranyl diphosphate wild-type, 30°C, pH not specified in the publication Picea sitchensis

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Picea sitchensis 9507
-

Organism

Organism UniProt Comment Textmining
Picea sitchensis F1CKI6 isoform TPS-3car1
-
Picea sitchensis F1CKI8 isoform TPS-3car2
-
Picea sitchensis F1CKI9 isoform TPS-3car3
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geranyl diphosphate
-
 Picea sitchensis (+)-car-3-ene + diphosphate wild-type produces 46.2% (+)-car-3-ene, 8.8% (-)-sabinene, 29.7% terpinolene ?
geranyl diphosphate
-
 Picea sitchensis (+)-car-3-ene + diphosphate wild-type produces 49% (+)-car-3-ene, 8.7% (-)-sabinene, 24.7% terpinolene ?
geranyl diphosphate
-
 Picea sitchensis (+)-car-3-ene + diphosphate wild-type produces 67.5% (+)-car-3-ene, 6.9% (-)-sabinene, 15.4% terpinolene ?

Synonyms

Synonyms Comment Organism
(+)-3-carene synthase
-
 Picea sitchensis
TPS-3car1
-
 Picea sitchensis
TPS-3car2
-
 Picea sitchensis
TPS-3car3
-
 Picea sitchensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.098
-
 geranyl diphosphate wild-type, 30°C, pH not specified in the publication Picea sitchensis
0.643
-
 geranyl diphosphate mutant L596F, 30°C, pH not specified in the publication Picea sitchensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0062
-
 geranyl diphosphate wild-type, 30°C, pH not specified in the publication Picea sitchensis
0.042
-
 geranyl diphosphate mutant L596F, 30°C, pH not specified in the publication Picea sitchensis