Cloned (Comment) | Organism |
---|---|
gene ZSSI, recombinant coexpression of alpha-humulene synthase of Zingiber zerumbet with alpha-humulene 8-hydroxylase (CYP71BA1) and zerumbone synthase variant (ZSD1S114A) from Zingiber zerumbet, together with AtCPR1 from Arabidopsis thaliana in Saccharomyces cerevisiae strain LW16, subcloning and expression in Escherichia coli strain trans-T1 | Zingiber zerumbet |
Protein Variants | Comment | Organism |
---|---|---|
additional information | de novo production of zerumbone is achieved in a metabolically engineered yeast cell factory by introducing alpha-humulene synthase (ZSS1), alpha-humulene 8-hydroxylase (CYP71BA1) and zerumbone synthase variant (ZSD1S114A) from Zingiber zerumbet, together with AtCPR1 from Arabidopsis thaliana into the Saccharomyces cerevisiae strain. Multistep metabolic engineering strategies are applied, including the overexpression of the mevalonate (MVA) pathway rate-limiting enzymes tHMG1 and yeast ERG20, regulation of yeast ERG9 by an inducible promoter and competitive pathway deletion to redirect metabolic flux toward the desired product, method evaluation and optimization, overview. In the engineered strain, alpha-humulene production increases by 18fold, to 92 mg/l compared to that in the original strain, fed-batch fermentation using Saccharomyces cerevisiae strain LW16 in a 5-l bioreactor | Zingiber zerumbet |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate | Zingiber zerumbet | - |
alpha-humulene + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zingiber zerumbet | B1B1U3 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate | - |
Zingiber zerumbet | alpha-humulene + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ZSS1 | - |
Zingiber zerumbet |