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Literature summary for 4.2.3.104 extracted from

  • Kumeta, Y.; Ito, M.
    Characterization of alpha-humulene synthases responsible for the production of sesquiterpenes induced by methyl jasmonate in Aquilaria cell culture (2016), J. Nat. Med., 70, 452-459 .
    View publication on PubMed
Search for more literature for 4.2.3.104

Cloned(Commentary)

Cloned (Comment) Organism
gene HS1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR expression analysis, heterologous expression of the enzyme in Escherichia coli Aquilaria crassna
gene HS2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR expression analysis, heterologous expression of the enzyme in Escherichia coli Aquilaria crassna
gene HS3, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR expression analysis, heterologous expression of the enzyme in Escherichia coli Aquilaria crassna

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information the enzyme has no N-terminal transit peptide Aquilaria crassna
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2E,6E)-farnesyl diphosphate Aquilaria crassna
-
 alpha-humulene + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Aquilaria crassna A0A142F308
-
-
Aquilaria crassna A0A142F309
-
-
Aquilaria crassna A0A142F310
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information the enzyme has no N-terminal transit peptide Aquilaria crassna

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate
-
 Aquilaria crassna alpha-humulene + diphosphate
-
 ?
additional information the recombinant alpha-humulene synthase isozyme HS1 produces 94.6% alpha-humulene and 5.4% beta-caryophyllene Aquilaria crassna ?
-
 ?
additional information the recombinant alpha-humulene synthase isozyme HS2 produces 95.0% alpha-humulene and 5.0% beta-caryophyllene Aquilaria crassna ?
-
 ?
additional information the recombinant alpha-humulene synthase isozyme HS3 produces 93.5% alpha-humulene and 6.5% beta-caryophyllene Aquilaria crassna ?
-
 ?

Synonyms

Synonyms Comment Organism
Hs1
-
 Aquilaria crassna
Hs2
-
 Aquilaria crassna
HS3
-
 Aquilaria crassna

Expression

Organism Comment Expression
Aquilaria crassna methyl jasmonate slightly induces the expression of enzyme alpha-humulene synthase, at 6 h after methyl jasmonate treatment, the expression of the alpha-humulene synthase gene has increased approximately 1.5fold resulting in 330 pmol/mg protein/h alpha-humulene production up

General Information

General Information Comment Organism
metabolism the enzyme is involved in the biosynthetic pathways for sesquiterpenes found in agarwood and cell suspension cultures, putative pathway, overview. Various post-transcriptional, translational, or post-translational regulatory mechanisms affecting the expression or activity of alpha-humulene and delta-guaiene synthases are possible Aquilaria crassna
additional information the enzyme contains conserved RPx8W motif and DDxxD motif, which is a divalent metal-ion substrate-binding site Aquilaria crassna