BRENDA - Enzyme Database show
show all sequences of 4.2.3.104

CO2 to terpenes autotrophic and electroautotrophic alpha-humulene production with Cupriavidus necator

Krieg, T.; Sydow, A.; Faust, S.; Huth, I.; Holtmann, D.; Angew. Chem. Int. Ed. Engl. 57, 1879-1882 (2018)

Data extracted from this reference:

Application
Application
Commentary
Organism
synthesis
chemolithoautotrophic production of a terpene from carbon dioxide, hydrogen, and oxygen, involving the engineered Cupriavidus necator cells and enzyme alpha-humulene synthase, is a promising starting point for the production of different high-value terpene compounds from abundant and simple raw materials. The production system is used to produce 17 mg alpha-humulene per gram cell dry mass (CDW) from CO2 and electrical energy in microbial electrosynthesis (MES) mode
Cupriavidus necator
Engineering
Amino acid exchange
Commentary
Organism
additional information
heterologous expression of the mevalonate pathway and alpha-humulene synthase results in the production of alpha-humulene from CO2 under heterotrophic conditions. The production system is used to produce 17 mg alpha-humulene per gram cell dry mass (CDW) from CO2 and electrical energy in microbial electrosynthesis (MES) mode
Cupriavidus necator
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(2E,6E)-farnesyl diphosphate
Cupriavidus necator
-
alpha-humulene + diphosphate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Cupriavidus necator
-
i.e. Ralstonia eutropha or Alcaligenes eutrophus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2E,6E)-farnesyl diphosphate
-
746751
Cupriavidus necator
alpha-humulene + diphosphate
-
-
-
?
Application (protein specific)
Application
Commentary
Organism
synthesis
chemolithoautotrophic production of a terpene from carbon dioxide, hydrogen, and oxygen, involving the engineered Cupriavidus necator cells and enzyme alpha-humulene synthase, is a promising starting point for the production of different high-value terpene compounds from abundant and simple raw materials. The production system is used to produce 17 mg alpha-humulene per gram cell dry mass (CDW) from CO2 and electrical energy in microbial electrosynthesis (MES) mode
Cupriavidus necator
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
heterologous expression of the mevalonate pathway and alpha-humulene synthase results in the production of alpha-humulene from CO2 under heterotrophic conditions. The production system is used to produce 17 mg alpha-humulene per gram cell dry mass (CDW) from CO2 and electrical energy in microbial electrosynthesis (MES) mode
Cupriavidus necator
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(2E,6E)-farnesyl diphosphate
Cupriavidus necator
-
alpha-humulene + diphosphate
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2E,6E)-farnesyl diphosphate
-
746751
Cupriavidus necator
alpha-humulene + diphosphate
-
-
-
?
Other publictions for EC 4.2.3.104
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746751
Krieg
CO2 to terpenes autotrophic a ...
Cupriavidus necator
Angew. Chem. Int. Ed. Engl.
57
1879-1882
2018
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748561
Zhang
Production of sesquiterpenoid ...
Zingiber zerumbet
Metab. Eng.
49
28-35
2018
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747656
Alemdar
-
Bioproduction of alpha-humule ...
Zingiber zerumbet
Eng. Life Sci.
17
900-907
2017
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1
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4
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1
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1
1
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746789
Alemdar
Heterologous expression, puri ...
Zingiber zerumbet
Appl. Biochem. Biotechnol.
178
474-489
2016
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1
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2
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748440
Kumeta
Characterization of alpha-hum ...
Aquilaria crassna
J. Nat. Med.
70
452-459
2016
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6
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1
2
6
3
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748552
Sonntag
Engineering Methylobacterium ...
Zingiber zerumbet
Metab. Eng.
32
82-94
2015
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730575
Yang
Isolation and characterization ...
Gossypium hirsutum
Phytochemistry
96
46-56
2013
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716562
Bleeker
RNA-seq discovery, functional ...
Solanum habrochaites
Plant Mol. Biol.
77
323-336
2011
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717468
Yu
Zingiber zerumbet CYP71BA1 cat ...
Zingiber zerumbet
Cell. Mol. Life Sci.
68
1033-1040
2011
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1
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1
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1
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716459
Koepke
Species-specific responses of ...
Pinus sylvestris
Phytochemistry
71
909-917
2010
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8
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718233
Schilmiller
Studies of a biochemical facto ...
Solanum lycopersicum
Plant Physiol.
153
1212-1223
2010
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690593
Harada
Efficient synthesis of functio ...
Zingiber zerumbet
Appl. Microbiol. Biotechnol.
81
915-925
2008
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1
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6
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1
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1
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718244
Yu
Molecular cloning and function ...
Zingiber zerumbet
Planta
227
1291-1299
2008
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1
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3
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1
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3
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1
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1
1
1
1
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713266
van Der Hoeven
Genetic control and evolution ...
no activity in Solanum habrochaites, Solanum lycopersicum
Plant Cell
12
2283-2294
2000
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4
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