Literature summary for 4.2.3.104 extracted from

Yu, F.; Okamto, S.; Nakasone, K.; Adachi, K.; Matsuda, S.; Harada, H.; Misawa, N.; Utsumi, R.
Molecular cloning and functional characterization of alpha-humulene synthase, a possible key enzyme of zerumbone biosynthesis in shampoo ginger (Zingiber zerumbet Smith) (2008), Planta, 227, 1291-1299.

Search for more literature for 4.2.3.104

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21-CodonPlus (DE3) cells	Zingiber zerumbet

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
64500	-	x * 64500, calculated from amino acid sequence	Zingiber zerumbet

Organism

Organism	UniProt	Comment	Textmining
Zingiber zerumbet	-	shampoo ginger	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography, gel filtration	Zingiber zerumbet

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	-	Zingiber zerumbet	-
additional information	not detected in stem	Zingiber zerumbet	-
rhizome	highest expression in rhizomes	Zingiber zerumbet	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(2E,6E)-farnesyl diphosphate	-	Zingiber zerumbet	alpha-humulene + diphosphate	95% yield of alpha-humulene besides the minor product beta-caryophyllene (5%)	?

Subunits

Subunits	Comment	Organism
?	x * 64500, calculated from amino acid sequence	Zingiber zerumbet

Synonyms

Synonyms	Comment	Organism
alpha-humulene synthase	-	Zingiber zerumbet
ZSS1	-	Zingiber zerumbet

pI Value

Organism	Comment	pI Value Maximum	pI Value
Zingiber zerumbet	calculated from amino acid sequence	-	5.3

Expression

Organism	Comment	Expression
Zingiber zerumbet	the enzyme is up-regulated in both leaves and rhizomes after treatment with methyl jasmonate	up

General Information

General Information	Comment	Organism
metabolism	alpha-humulene synthase is a key enzyme of zerumbone biosynthesis	Zingiber zerumbet

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Release 2023.1 (January 2023)