BRENDA - Enzyme Database show
show all sequences of 4.2.3.104

Molecular cloning and functional characterization of alpha-humulene synthase, a possible key enzyme of zerumbone biosynthesis in shampoo ginger (Zingiber zerumbet Smith)

Yu, F.; Okamto, S.; Nakasone, K.; Adachi, K.; Matsuda, S.; Harada, H.; Misawa, N.; Utsumi, R.; Planta 227, 1291-1299 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21-CodonPlus (DE3) cells
Zingiber zerumbet
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
64500
-
x * 64500, calculated from amino acid sequence
Zingiber zerumbet
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Zingiber zerumbet
-
shampoo ginger
-
Purification (Commentary)
Commentary
Organism
Ni-NTA column chromatography, gel filtration
Zingiber zerumbet
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Zingiber zerumbet
-
additional information
not detected in stem
Zingiber zerumbet
-
rhizome
highest expression in rhizomes
Zingiber zerumbet
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2E,6E)-farnesyl diphosphate
-
718244
Zingiber zerumbet
alpha-humulene + diphosphate
95% yield of alpha-humulene besides the minor product beta-caryophyllene (5%)
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 64500, calculated from amino acid sequence
Zingiber zerumbet
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Zingiber zerumbet
calculated from amino acid sequence
-
5.3
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21-CodonPlus (DE3) cells
Zingiber zerumbet
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
64500
-
x * 64500, calculated from amino acid sequence
Zingiber zerumbet
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-NTA column chromatography, gel filtration
Zingiber zerumbet
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Zingiber zerumbet
-
additional information
not detected in stem
Zingiber zerumbet
-
rhizome
highest expression in rhizomes
Zingiber zerumbet
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2E,6E)-farnesyl diphosphate
-
718244
Zingiber zerumbet
alpha-humulene + diphosphate
95% yield of alpha-humulene besides the minor product beta-caryophyllene (5%)
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 64500, calculated from amino acid sequence
Zingiber zerumbet
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Zingiber zerumbet
calculated from amino acid sequence
-
5.3
Expression
Organism
Commentary
Expression
Zingiber zerumbet
the enzyme is up-regulated in both leaves and rhizomes after treatment with methyl jasmonate
up
General Information
General Information
Commentary
Organism
metabolism
alpha-humulene synthase is a key enzyme of zerumbone biosynthesis
Zingiber zerumbet
General Information (protein specific)
General Information
Commentary
Organism
metabolism
alpha-humulene synthase is a key enzyme of zerumbone biosynthesis
Zingiber zerumbet
Expression (protein specific)
Organism
Commentary
Expression
Zingiber zerumbet
the enzyme is up-regulated in both leaves and rhizomes after treatment with methyl jasmonate
up
Other publictions for EC 4.2.3.104
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746751
Krieg
CO2 to terpenes autotrophic a ...
Cupriavidus necator
Angew. Chem. Int. Ed. Engl.
57
1879-1882
2018
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748561
Zhang
Production of sesquiterpenoid ...
Zingiber zerumbet
Metab. Eng.
49
28-35
2018
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1
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1
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747656
Alemdar
-
Bioproduction of alpha-humule ...
Zingiber zerumbet
Eng. Life Sci.
17
900-907
2017
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1
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4
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1
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3
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4
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1
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3
1
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1
1
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746789
Alemdar
Heterologous expression, puri ...
Zingiber zerumbet
Appl. Biochem. Biotechnol.
178
474-489
2016
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1
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1
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2
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1
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1
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1
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2
1
1
1
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1
1
1
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2
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2
2
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748440
Kumeta
Characterization of alpha-hum ...
Aquilaria crassna
J. Nat. Med.
70
452-459
2016
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1
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1
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3
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3
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1
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6
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6
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1
2
6
3
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748552
Sonntag
Engineering Methylobacterium ...
Zingiber zerumbet
Metab. Eng.
32
82-94
2015
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1
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1
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730575
Yang
Isolation and characterization ...
Gossypium hirsutum
Phytochemistry
96
46-56
2013
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1
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2
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5
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7
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2
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1
1
1
2
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716562
Bleeker
RNA-seq discovery, functional ...
Solanum habrochaites
Plant Mol. Biol.
77
323-336
2011
-
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1
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1
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717468
Yu
Zingiber zerumbet CYP71BA1 cat ...
Zingiber zerumbet
Cell. Mol. Life Sci.
68
1033-1040
2011
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1
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1
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3
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3
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1
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1
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-
716459
Koepke
Species-specific responses of ...
Pinus sylvestris
Phytochemistry
71
909-917
2010
-
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8
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1
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1
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718233
Schilmiller
Studies of a biochemical facto ...
Solanum lycopersicum
Plant Physiol.
153
1212-1223
2010
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1
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1
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690593
Harada
Efficient synthesis of functio ...
Zingiber zerumbet
Appl. Microbiol. Biotechnol.
81
915-925
2008
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1
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6
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1
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718244
Yu
Molecular cloning and function ...
Zingiber zerumbet
Planta
227
1291-1299
2008
-
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1
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1
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3
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1
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3
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1
1
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1
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1
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1
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1
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3
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1
1
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1
1
1
1
1
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713266
van Der Hoeven
Genetic control and evolution ...
no activity in Solanum habrochaites, Solanum lycopersicum
Plant Cell
12
2283-2294
2000
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1
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4
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1
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1
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