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Literature summary for 4.2.3.102 extracted from

  • Koellner, T.; Schnee, C.; Gershenzon, J.; Degenhardt, J.
    The variability of sesquiterpenes emitted from two Zea mays cultivars is controlled by allelic variation of two terpene synthase genes encoding stereoselective multiple product enzymes (2004), Plant Cell, 16, 1115-1131.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.3.102

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Zea mays

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ either Mg2+ or Mn2+ is required for activity, the optimum is 5 mM Mg2+ Zea mays
Mn2+ either Mg2+ or Mn2+ is required for activity, the optimum is 0.25 mM Mg2+ Zea mays
additional information no enzyme activity in the presence of Ca2+, Cd2+, Co2+, Cu2+, Ni2+, and Zn2+ Zea mays

Organism

Organism UniProt Comment Textmining
Zea mays
-
 variety Delprim
-

Purification (Commentary)

Purification (Comment) Organism
Econopac 10DG column chromatography and Strep-Tactine affinity column chromatography Zea mays

Source Tissue

Source Tissue Comment Organism Textmining
husk highest expression Zea mays
-
leaf ear leaf Zea mays
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate
-
 Zea mays sesquithujene + diphosphate sesquithujene is the major product, the enzyme also produces (S)-beta-bisabolene, and the minor products 7-epi-sesquithujene, (Z)-alpha-bergamotene, (E)-alpha-bergamotene, sesquisabinene B, sesquisabinene A, and (E)-beta-farnesene ?
geranyl diphosphate
-
 Zea mays (S)-(-)-limonene + diphosphate main product is (S)-(-)-limonene, minor products are alpha-thujene, beta-myrcene, (+)-sabinene, (-)-sabinene, alpha-terpinene, (E)-beta-ocimene, beta-phellandrene, alpha-terpinolene, gamma-terpinene, sabinene hydrate, (R)-(-)-linalool, and (S)-(1)-linalool ?

Synonyms

Synonyms Comment Organism
TPS5
-
 Zea mays
TPS5-Del1
-
 Zea mays