Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE) cells | Zea mays |
Protein Variants | Comment | Organism |
---|---|---|
E391Q | the mutation is located in the vicinity of the catalytic center but does not influence enzymatic activity | Zea mays |
E455V | the mutation results in an increase of catalytic activity | Zea mays |
I479T | the mutation is located in the vicinity of the catalytic center but does not influence enzymatic activity | Zea mays |
K402Q | the mutation is located in the vicinity of the catalytic center but does not influence enzymatic activity | Zea mays |
T383S | the mutation is located in the vicinity of the catalytic center but does not influence enzymatic activity | Zea mays |
W163R | the mutation restores much of the wild type activity (albeit maintaining the increased concentration of (E)-beta-farnesene relative to that of sesquithujene and (S)-beta-bisabolene) | Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0009 | - |
geranyl diphosphate | 20 mM MgCl2, 0.2 mM MnCl2, 0.2 mM NaWO4, and 0.1 mM NaF, pH not specified in the publication, at 30°C | Zea mays | |
0.0011 | - |
(2E,6E)-farnesyl diphosphate | 20 mM MgCl2, 0.2 mM MnCl2, 0.2 mM NaWO4, and 0.1 mM NaF, pH not specified in the publication, at 30°C | Zea mays |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | either Mg2+ or Mn2+ is required for activity, the optimum is 5 mM Mg2+ | Zea mays | |
Mn2+ | either Mg2+ or Mn2+ is required for activity, the optimum is 0.25 mM Mg2+ | Zea mays | |
additional information | no enzyme activity in the presence of Ca2+, Cd2+, Co2+, Cu2+, Ni2+, and Zn2+ | Zea mays |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63960 | - |
calculated from amino acid sequence | Zea mays |
66000 | - |
gel filtration | Zea mays |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | - |
inbred line B73 | - |
Purification (Comment) | Organism |
---|---|
Econopac 10DG column chromatography and Strep-Tactine affinity column chromatography | Zea mays |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
husk | highest expression | Zea mays | - |
leaf | ear leaf | Zea mays | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate | - |
Zea mays | 7-epi-sesquithujene + diphosphate | 7-epi-sesquithujene is the main product, the enzyme also produces (S)-beta-bisabolene, (E)-beta-farnesene, and the minor products sesquithujene, (Z)-alpha-bergamotene, sesquisabinene B, (E)-alpha-bergamotene, and sesquisabinene A | ? | |
geranyl diphosphate | - |
Zea mays | (S)-(-)-limonene + diphosphate | main product is (S)-(-)-limonene, minor products are alpha-thujene, beta-myrcene, (+)-sabinene, (-)-sabinene, alpha-terpinene, (E)-beta-ocimene, beta-phellandrene, alpha-terpinolene, gamma-terpinene, sabinene hydrate, (R)-(-)-linalool, and (S)-(1)-linalool | ? |
Synonyms | Comment | Organism |
---|---|---|
TPS4 | - |
Zea mays |
TPS4-B73 | - |
Zea mays |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Zea mays |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.3 | 8.2 | - |
Zea mays |