Literature summary for 4.2.3.1 extracted from

Covarrubias, A.S.; H�gbom, M.; Bergfors, T.; Carroll, P.; Mannerstedt, K.; Oscarson, S.; Parish, T.; Jones, T.A.; Mowbray, S.L.
Structural, biochemical, and in vivo investigations of the threonine synthase from Mycobacterium tuberculosis (2008), J. Mol. Biol., 381, 622-633.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed as a His-tagged fusion protein	Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment)	Organism
refined to 2.5 A. The structure of MtTS has a homodimeric organization in which the two subunits are related by a non-crystallographic 2fold axis. Each subunit is composed of three domains	Mycobacterium tuberculosis

Protein Variants

Protein Variants	Comment	Organism
additional information	a deletion of the MtTS gene generated a strain of Mycobacterium tuberculosis that requires threonine for growth	Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.2	-	O-phospho-L-homoserine	-	Mycobacterium tuberculosis

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	P9WG59	-	-
Mycobacterium tuberculosis H37Rv	P9WG59	-	-

Purification (Commentary)

Purification (Comment)	Organism
using Ni-NTA chromatography	Mycobacterium tuberculosis

Reaction

Reaction	Comment	Organism	Reaction ID
O-phospho-L-homoserine + H2O = L-threonine + phosphate	analyzed crystal structure gives new insights into the catalytic mechanism of threonine synthase in general, specifically by suggesting the direct involvement of the phosphate moiety of the cofactor, rather than the inorganic phosphate product, in transferring a proton from C4 to Cgamma in the formation of the alphabeta-unsaturated aldimine	Mycobacterium tuberculosis	a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.05	-	-	Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
O-phospho-L-homoserine + H2O	-	Mycobacterium tuberculosis	L-threonine + phosphate	-	?
O-phospho-L-homoserine + H2O	-	Mycobacterium tuberculosis H37Rv	L-threonine + phosphate	-	?

Subunits

Subunits	Comment	Organism
homodimer	crystal structure	Mycobacterium tuberculosis

Synonyms

Synonyms	Comment	Organism
MtTS	-	Mycobacterium tuberculosis
threonine synthase	-	Mycobacterium tuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Mycobacterium tuberculosis

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
60	-	MtTS loses less than 10% of the initial activity during a 10 min incubation	Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
7.3	-	O-phospho-L-homoserine	-	Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.4	-	assay at	Mycobacterium tuberculosis

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
7	-	MtTS is inactivated at pH 7 or below	Mycobacterium tuberculosis

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	MtTS is not influenced by S-adenosylmethionine	Mycobacterium tuberculosis

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Release 2023.1 (January 2023)