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Literature summary for 4.2.2.B7 extracted from

  • Ochiai, A.; Hashimoto, W.; Murata, K.
    A biosystem for alginate metabolism in Agrobacterium tumefaciens strain C58 molecular identification of Atu3025 as an exotype family PL-15 alginate lyase (2006), Res. Microbiol., 157, 642-649 .
    View publication on PubMed
Search for more literature for 4.2.2.B7

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Agrobacterium tumefaciens

Inhibitors

Inhibitors Comment Organism Structure
galacturonic acid 1 mM, 93% inhibition Agrobacterium tumefaciens
glucuronic acid 1 mM, 38% inhibition Agrobacterium tumefaciens
Hg2+ 1 mM, 96% inhibition Agrobacterium tumefaciens
additional information not inhibitory: Co2+, Mg2+, Mn2+, dithiothreitol, N-ethylmaleimide, 2-mercaptoethanol, iodoacetic acid, p-chloromercuribenzoate Agrobacterium tumefaciens
Zn2+ 1 mM, 100% inhibition Agrobacterium tumefaciens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0094
-
 alginate pH 7.5, 30°C Agrobacterium tumefaciens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
350000
-
 gel filtration Agrobacterium tumefaciens

Organism

Organism UniProt Comment Textmining
Agrobacterium tumefaciens A9CEJ9
-
-
Agrobacterium tumefaciens C58/ATCC 33970 A9CEJ9
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
15.3
-
 substrate poly(alpha-(1,4)-L-guluronate), pH 7.5, 30°C Agrobacterium tumefaciens
16.5
-
 substrate poly(beta-(1,4)-D-mannuronate), pH 7.5, 30°C Agrobacterium tumefaciens
18
-
 substrate poly(beta-1,4-D-mannuronate/alpha-1,4-L-guluronate), pH 7.5, 30°C Agrobacterium tumefaciens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alginate
-
 Agrobacterium tumefaciens ?
-
 ?
alginate
-
 Agrobacterium tumefaciens C58/ATCC 33970 ?
-
 ?
additional information Atu3025 releases monosaccharides specifically from alginate through a beta-elimination reaction Agrobacterium tumefaciens ?
-
 ?
additional information Atu3025 releases monosaccharides specifically from alginate through a beta-elimination reaction Agrobacterium tumefaciens C58/ATCC 33970 ?
-
 ?
poly(alpha-(1,4)-L-guluronate)
-
 Agrobacterium tumefaciens 4-deoxy-alpha-L-erythro-hex-4-enopyranuronic acid
-
 ?
poly(alpha-(1,4)-L-guluronate)
-
 Agrobacterium tumefaciens C58/ATCC 33970 4-deoxy-alpha-L-erythro-hex-4-enopyranuronic acid
-
 ?
poly(beta-(1,4)-D-mannuronate)
-
 Agrobacterium tumefaciens 4-deoxy-alpha-L-erythro-hex-4-enopyranuronic acid
-
 ?
poly(beta-(1,4)-D-mannuronate)
-
 Agrobacterium tumefaciens C58/ATCC 33970 4-deoxy-alpha-L-erythro-hex-4-enopyranuronic acid
-
 ?
poly(beta-1,4-D-mannuronate/alpha-1,4-L-guluronate)
-
 Agrobacterium tumefaciens 4-deoxy-alpha-L-erythro-hex-4-enopyranuronic acid + 4-deoxy-alpha-L-erythro-hex-4-enopyranuronic acid
-
 ?
poly(beta-1,4-D-mannuronate/alpha-1,4-L-guluronate)
-
 Agrobacterium tumefaciens C58/ATCC 33970 4-deoxy-alpha-L-erythro-hex-4-enopyranuronic acid + 4-deoxy-alpha-L-erythro-hex-4-enopyranuronic acid
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 88000, SDS-PAGE, 4 * 87871, calculated from sequence Agrobacterium tumefaciens

Synonyms

Synonyms Comment Organism
Atu 3025
-
 Agrobacterium tumefaciens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Agrobacterium tumefaciens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
-
 Agrobacterium tumefaciens

Expression

Organism Comment Expression
Agrobacterium tumefaciens expression is induced by growth on low-molecular-weight alginate up