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Literature summary for 4.2.2.8 extracted from
- Expanding the catalytic promiscuity of heparinase III from Pedobacter heparinus (2017), Chemistry, 23, 2548-2551 .
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| G112C | loss of activity | Pedobacter heparinus |
| K130C | mutant is able to degrade hyaluronic acid without affecting its native activity toward heparin and heparan sulfate, due to dimerization through a disulfide bond to expand the substrate binding pocket | Pedobacter heparinus |
| Q89C | loss of activity | Pedobacter heparinus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.08 | - |
heparan sulfate | wild-type, pH not specified in the publication, temperature not specified in the publication | Pedobacter heparinus |  |
| 0.093 | - |
heparan sulfate | mutant K130C, pH not specified in the publication, temperature not specified in the publication | Pedobacter heparinus | |
| 0.326 | - |
heparin | wild-type, pH not specified in the publication, temperature not specified in the publication | Pedobacter heparinus | |
| 0.344 | - |
heparin | mutant K130C, pH not specified in the publication, temperature not specified in the publication | Pedobacter heparinus | |
| 0.438 | - |
hyaluronate | mutant K130C, pH not specified in the publication, temperature not specified in the publication | Pedobacter heparinus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 130000 | - |
dynamic light scattering, mutant K130C | Pedobacter heparinus |
| 133900 | - |
gel fitlration, mutant K130C | Pedobacter heparinus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pedobacter heparinus | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| heparan sulfate | - |
Pedobacter heparinus | unsaturated heparan disaccharide | - |
? | |
| heparin | - |
Pedobacter heparinus | unsaturated heparin disaccharide + ? | - |
? | |
| hyaluronate | no substrate of wild-type, but substrate of mutant K130C. Reaction of EC 4.2.2.1 | Pedobacter heparinus | unsaturated hyaluronate disaccharide | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.3 | - |
hyaluronate | mutant K130C, pH not specified in the publication, temperature not specified in the publication | Pedobacter heparinus | |
| 1.6 | - |
heparin | mutant K130C, pH not specified in the publication, temperature not specified in the publication | Pedobacter heparinus | |
| 1.9 | - |
heparin | wild-type, pH not specified in the publication, temperature not specified in the publication | Pedobacter heparinus | |
| 56 | - |
heparan sulfate | mutant K130C, pH not specified in the publication, temperature not specified in the publication | Pedobacter heparinus | |
| 68 | - |
heparan sulfate | wild-type, pH not specified in the publication, temperature not specified in the publication | Pedobacter heparinus | |
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