show reference and datareference onlydata only

Cloning, overexpression, and characterization of recombinant heparinase III from Bacteroides stercoris HJ-15

Data extracted from this reference:

Inhibitors

Commentary

Organism

Structure

Cu2+

1 mM: 0% activity

Bacteroides stercoris

Pb2+

1 mM: 0% activity

Bacteroides stercoris

Zn2+

1 mM: 0% activity

Bacteroides stercoris

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

additional information

-

heparan sulfate

0.08808 mg/ml

Bacteroides stercoris

additional information

-

heparin

0.42275 mg/ml

Bacteroides stercoris

Metals/Ions

Commentary

Organism

Structure

Ba2+

1 mM: 109% activity

Bacteroides stercoris

Ca2+

1 mM: 110% activity

Bacteroides stercoris

additional information

optimal condition: 350 mM NaCl

Bacteroides stercoris

Inhibitors

Commentary

Organism

Structure

Cu2+

1 mM: 0% activity

Bacteroides stercoris

Pb2+

1 mM: 0% activity

Bacteroides stercoris

Zn2+

1 mM: 0% activity

Bacteroides stercoris

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

additional information

-

heparan sulfate

0.08808 mg/ml

Bacteroides stercoris

additional information

-

heparin

0.42275 mg/ml

Bacteroides stercoris

Metals/Ions

Commentary

Organism

Structure

Ba2+

1 mM: 109% activity

Bacteroides stercoris

Ca2+

1 mM: 110% activity

Bacteroides stercoris

additional information

optimal condition: 350 mM NaCl

Bacteroides stercoris

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

748820

Gu

Photoswitchable heparinase II ...

Pedobacter heparinus

Org. Lett.

20

48-51

2018

-

1

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

747456

Hu

Probing cleavage promiscuity ...

Pedobacter heparinus

Carbohydr. Polym.

173

276-285

2017

-

-

1

-

-

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

-

-

16

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

16

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

747570

Gu

Expanding the catalytic promi ...

Pedobacter heparinus

Chemistry

23

2548-2551

2017

-

-

-

-

3

-

-

5

-

-

2

-

-

1

-

-

-

-

-

-

-

-

3

-

-

-

-

5

-

-

-

-

-

-

-

-

-

-

-

-

3

-

-

-

-

5

-

-

2

-

-

-

-

-

-

-

-

-

3

-

-

-

-

5

-

-

-

-

-

-

-

-

-

-

747916

Ulaganathan

Conformational flexibility of ...

Bacteroides thetaiotaomicron, Bacteroides thetaiotaomicron DSM 2079

Glycobiology

27

176-187

2017

-

-

1

1

3

-

-

1

-

-

-

-

-

4

-

-

-

-

-

-

-

-

11

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

1

3

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

11

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

747454

Carnachan

Determining the extent of hep ...

Pedobacter heparinus

Carbohydr. Polym.

152

592-597

2016

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

729269

Hashimoto

Crystal structure of Pedobacte ...

Pedobacter heparinus, Pedobacter heparinus DSM 2366

Biochemistry

53

777-786

2014

-

-

-

1

11

-

-

-

-

-

-

-

-

4

-

-

-

-

-

-

-

-

-

-

-

-

6

-

-

-

-

-

-

-

-

-

-

-

-

1

11

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

6

-

-

-

-

-

-

-

-

-

-

-

730858

Dong

Structural basis of heparan su ...

Bacteroides thetaiotaomicron, Bacteroides thetaiotaomicron DSM 2079

Protein Cell

3

950-961

2012

-

-

1

1

-

-

-

-

-

-

-

-

-

3

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

714553

Chen

Biochemical analysis and kinet ...

Pedobacter heparinus

Biotechnol. Bioeng.

108

1841-1851

2011

-

-

1

-

1

1

11

1

-

1

-

-

-

1

-

-

1

-

1

-

1

1

1

1

1

-

3

-

1

-

-

-

-

-

-

-

-

1

-

-

1

1

-

11

-

1

-

1

-

-

-

-

-

1

1

-

1

1

1

1

1

-

3

-

1

-

-

-

-

1

1

-

-

-

701676

Babu

Fluorescent-tagged heparan sul ...

Pedobacter heparinus

Anal. Biochem.

396

124-132

2010

-

-

1

-

-

-

-

-

-

-

-

-

-

1

-

-

1

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

701885

Hyun

Cloning, overexpression, and c ...

Bacteroides stercoris, Bacteroides stercoris HJ-15

Appl. Microbiol. Biotechnol.

86

879-890

2010

-

-

1

-

4

-

3

2

-

3

1

-

-

5

-

-

1

-

-

-

5

-

6

1

1

1

1

-

1

1

-

-

-

1

-

-

-

1

-

-

4

-

-

3

-

2

-

3

1

-

-

-

-

1

-

-

5

-

6

1

1

1

1

-

1

1

-

1

-

-

-

-

-

-

714027

Raman

Differential effects of hepari ...

Pedobacter heparinus

Biochem. Biophys. Res. Commun.

398

191-193

2010

-

-

-

-

-

-

-

-

-

-

-

-

-

3

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

1

1

-

-

-

682098

Rops

Heparan sulfate on activated g ...

Pedobacter heparinus

Nephrol. Dial. Transplant.

22

1070-1077

2007

-

-

-

-

-

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

665613

Wei

Distinct substrate specificiti ...

Pedobacter heparinus

J. Biol. Chem.

280

15742-15748

2005

-

-

-

-

-

-

-

-

-

-

-

-

-

3

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

664047

Chai

Relative susceptibilities of t ...

Pedobacter heparinus

Biochemistry

43

8590-8599

2004

-

-

-

-

-

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

649969

Pojasek

Histidine 295 and histidine 51 ...

Pedobacter heparinus

Biochemistry

39

4012-4019

2000

-

-

1

-

13

-

1

11

-

-

-

-

-

2

-

-

1

1

-

-

-

-

1

-

1

-

-

12

1

-

-

-

1

-

-

-

-

1

-

-

13

-

-

1

1

11

-

-

-

-

-

-

-

1

-

-

-

-

1

-

1

-

-

12

1

-

-

-

-

-

-

-

-

-

37411

Graham

Inhibition of platelet heparit ...

Homo sapiens

Biochem. Mol. Biol. Int.

37

239-246

1995

-

-

-

-

-

-

2

-

-

-

-

-

-

1

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

37409

Yamada

Structural studies on the tri- ...

Pedobacter heparinus

Glycobiology

4

69-78

1994

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

37410

Suhahara

Structural studies on the olig ...

Pedobacter heparinus

Glycobiology

4

535-544

1994

-

-

-

-

-

-

-

-

-

-

-

-

-

4

-

-

-

-

-

2

-

-

-

-

1

-

-

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1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

2

-

-

-

-

1

-

-

-

1

-

-

-

-

-

-

-

-

-

37412

Brickman

Heparitinase inhibition of mes ...

Pedobacter heparinus

Dev. Biol.

164

484-501

1994

-

1

-

-

-

-

-

-

-

-

-

-

-

4

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

37407

Desai

Specificity studies on the hep ...

Pedobacter heparinus

Biochemistry

32

8140-8145

1993

-

-

-

-

-

-

-

-

-

-

-

1

-

2

-

-

1

-

-

-

-

-

5

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

1

-

-

-

-

5

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

37406

Nader H.B.; Porcionatto M.A.; Tersariol

Purification and substrate spe ...

Pedobacter heparinus

J. Biol. Chem.

265

16807-16813

1990

-

-

-

-

-

-

-

-

-

-

-

1

-

2

-

-

1

-

-

-

-

-

6

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

1

-

-

-

-

6

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

37408

Linhardt

Examination of the substrate s ...

Pedobacter heparinus

Biochemistry

29

2611-2617

1990

-

-

-

-

-

-

-

-

-

-

-

1

-

2

-

-

1

-

-

-

-

-

2

-

1

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

1

-

-

-

-

2

-

1

-

-

-

1

-

-

-

-

-

-

-

-

-

37404

Nader

Heparin sequences in the hepar ...

Pedobacter heparinus

Proc. Natl. Acad. Sci. USA

84

3565-3569

1987

-

-

-

-

-

-

-

-

-

-

-

1

-

2

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

37405

Prinz

Metabolism of sulfated glycosa ...

Rattus norvegicus

Biochim. Biophys. Acta

630

402-413

1980

-

-

-

-

-

-

-

-

-

-

-

1

-

1

-

-

-

-

-

1

-

-

2

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

1

-

-

2

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

5978

Hovingh

The enzymatic degradation of h ...

Flavobacteriia

J. Biol. Chem.

245

6170-6175

1970

-

-

-

-

-

-

5

-

-

3

-

-

-

2

-

-

1

-

-

-

-

1

1

-

1

1

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

5

-

-

-

3

-

-

-

-

-

1

-

-

-

1

1

-

1

1

-

-

1

-

-

-

-

-

-

-

-

-