Data extracted from this reference:
Cloned(Commentary)
His-tagged version expressed in Escherichia coli BL21(DE3)
Bacteroides stercoris
Engineering
C352A
87% wild-type activity (heparan sulfate), 72% wild-type activity (heparin)
Bacteroides stercoris
C474A
32% wild-type activity (heparan sulfate), below 10% wild-type activity (heparin)
Bacteroides stercoris
C577A
98% wild-type activity (heparan sulfate), 72% wild-type activity (heparin)
Bacteroides stercoris
C624A
24% wild-type activity (heparan sulfate), below 10% wild-type activity (heparin)
Bacteroides stercoris
Inhibitors
Cu2+
1 mM: 0% activity
Bacteroides stercoris
Pb2+
1 mM: 0% activity
Bacteroides stercoris
Zn2+
1 mM: 0% activity
Bacteroides stercoris
KM Value [mM]
additional information
heparan sulfate
0.08808 mg/ml
Bacteroides stercoris
additional information
heparin
0.42275 mg/ml
Bacteroides stercoris
Metals/Ions
Ba2+
1 mM: 109% activity
Bacteroides stercoris
Ca2+
1 mM: 110% activity
Bacteroides stercoris
additional information
optimal condition: 350 mM NaCl
Bacteroides stercoris
Molecular Weight [Da]
74930
x * 74930, calculated for mature protein
Bacteroides stercoris
Organism
Bacteroides stercoris
C7EXL6
Bacteroides stercoris HJ-15
C7EXL6
Purification (Commentary)
immobilized metal ion affinity chromatography
Bacteroides stercoris
Specific Activity [micromol/min/mg]
additional information
no activity with acharan sulfate, chondroitin sulfate A, and chondroitin sulfate C
Bacteroides stercoris
1.7
hyaluronic acid as substrate
Bacteroides stercoris
21.46
heparin as substrate
Bacteroides stercoris
32.3
heparan sulfate (porcine intestine) as substrate
Bacteroides stercoris
51.81
heparan sulfate (bovine kidney) as substrate
Bacteroides stercoris
Substrates and Products (Substrate)
heparan sulfate
701885
Bacteroides stercoris
n alpha-deltaUA-glcNAc
major product
?
heparan sulfate
701885
Bacteroides stercoris HJ-15
n alpha-deltaUA-glcNAc
major product
?
heparin
701885
Bacteroides stercoris
?
?
heparin
701885
Bacteroides stercoris HJ-15
?
?
hyaluronic acid
701885
Bacteroides stercoris
?
?
hyaluronic acid
701885
Bacteroides stercoris HJ-15
?
?
Subunits
?
x * 74930, calculated for mature protein
Bacteroides stercoris
Temperature Optimum [°C]
Temperature Range [°C]
42
48
activity above 90%
Bacteroides stercoris
Temperature Stability [°C]
additional information
about 20% activity after 1 h at 45°C
Bacteroides stercoris
pH Optimum
6.6
Bacteroides stercoris
pH Range
6
8
Bacteroides stercoris
pI Value
Bacteroides stercoris
calculated for mature protein
8.65
Cloned(Commentary) (protein specific)
His-tagged version expressed in Escherichia coli BL21(DE3)
Bacteroides stercoris
Engineering (protein specific)
C352A
87% wild-type activity (heparan sulfate), 72% wild-type activity (heparin)
Bacteroides stercoris
C474A
32% wild-type activity (heparan sulfate), below 10% wild-type activity (heparin)
Bacteroides stercoris
C577A
98% wild-type activity (heparan sulfate), 72% wild-type activity (heparin)
Bacteroides stercoris
C624A
24% wild-type activity (heparan sulfate), below 10% wild-type activity (heparin)
Bacteroides stercoris
Inhibitors (protein specific)
Cu2+
1 mM: 0% activity
Bacteroides stercoris
Pb2+
1 mM: 0% activity
Bacteroides stercoris
Zn2+
1 mM: 0% activity
Bacteroides stercoris
KM Value [mM] (protein specific)
additional information
heparan sulfate
0.08808 mg/ml
Bacteroides stercoris
additional information
heparin
0.42275 mg/ml
Bacteroides stercoris
Metals/Ions (protein specific)
Ba2+
1 mM: 109% activity
Bacteroides stercoris
Ca2+
1 mM: 110% activity
Bacteroides stercoris
additional information
optimal condition: 350 mM NaCl
Bacteroides stercoris
Molecular Weight [Da] (protein specific)
74930
x * 74930, calculated for mature protein
Bacteroides stercoris
Purification (Commentary) (protein specific)
immobilized metal ion affinity chromatography
Bacteroides stercoris
Specific Activity [micromol/min/mg] (protein specific)
additional information
no activity with acharan sulfate, chondroitin sulfate A, and chondroitin sulfate C
Bacteroides stercoris
1.7
hyaluronic acid as substrate
Bacteroides stercoris
21.46
heparin as substrate
Bacteroides stercoris
32.3
heparan sulfate (porcine intestine) as substrate
Bacteroides stercoris
51.81
heparan sulfate (bovine kidney) as substrate
Bacteroides stercoris
Substrates and Products (Substrate) (protein specific)
heparan sulfate
701885
Bacteroides stercoris
n alpha-deltaUA-glcNAc
major product
?
heparan sulfate
701885
Bacteroides stercoris HJ-15
n alpha-deltaUA-glcNAc
major product
?
heparin
701885
Bacteroides stercoris
?
?
heparin
701885
Bacteroides stercoris HJ-15
?
?
hyaluronic acid
701885
Bacteroides stercoris
?
?
hyaluronic acid
701885
Bacteroides stercoris HJ-15
?
?
Subunits (protein specific)
?
x * 74930, calculated for mature protein
Bacteroides stercoris
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
42
48
activity above 90%
Bacteroides stercoris
Temperature Stability [°C] (protein specific)
additional information
about 20% activity after 1 h at 45°C
Bacteroides stercoris
pH Optimum (protein specific)
6.6
Bacteroides stercoris
pH Range (protein specific)
6
8
Bacteroides stercoris
pI Value (protein specific)
Bacteroides stercoris
calculated for mature protein
8.65
Other publictions for EC 4.2.2.8
748820
Gu
Photoswitchable heparinase II ...
Pedobacter heparinus
Org. Lett.
20
48-51
2018
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
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-
-
-
-
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-
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-
-
-
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-
-
1
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
747456
Hu
Probing cleavage promiscuity ...
Pedobacter heparinus
Carbohydr. Polym.
173
276-285
2017
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
16
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
16
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
747570
Gu
Expanding the catalytic promi ...
Pedobacter heparinus
Chemistry
23
2548-2551
2017
-
-
-
-
3
-
-
5
-
-
2
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
5
-
-
2
-
-
-
-
-
-
-
-
-
3
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
747916
Ulaganathan
Conformational flexibility of ...
Bacteroides thetaiotaomicron, Bacteroides thetaiotaomicron DSM 2079
Glycobiology
27
176-187
2017
-
-
1
1
3
-
-
1
-
-
-
-
-
4
-
-
-
-
-
-
-
-
11
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
3
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
11
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
747454
Carnachan
Determining the extent of hep ...
Pedobacter heparinus
Carbohydr. Polym.
152
592-597
2016
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
729269
Hashimoto
Crystal structure of Pedobacte ...
Pedobacter heparinus, Pedobacter heparinus DSM 2366
Biochemistry
53
777-786
2014
-
-
-
1
11
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
1
11
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
730858
Dong
Structural basis of heparan su ...
Bacteroides thetaiotaomicron, Bacteroides thetaiotaomicron DSM 2079
Protein Cell
3
950-961
2012
-
-
1
1
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
714553
Chen
Biochemical analysis and kinet ...
Pedobacter heparinus
Biotechnol. Bioeng.
108
1841-1851
2011
-
-
1
-
1
1
11
1
-
1
-
-
-
1
-
-
1
-
1
-
1
1
1
1
1
-
3
-
1
-
-
-
-
-
-
-
-
1
-
-
1
1
-
11
-
1
-
1
-
-
-
-
-
1
1
-
1
1
1
1
1
-
3
-
1
-
-
-
-
1
1
-
-
-
701676
Babu
Fluorescent-tagged heparan sul ...
Pedobacter heparinus
Anal. Biochem.
396
124-132
2010
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
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-
-
-
-
-
1
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
701885
Hyun
Cloning, overexpression, and c ...
Bacteroides stercoris, Bacteroides stercoris HJ-15
Appl. Microbiol. Biotechnol.
86
879-890
2010
-
-
1
-
4
-
3
2
-
3
1
-
-
5
-
-
1
-
-
-
5
-
6
1
1
1
1
-
1
1
-
-
-
1
-
-
-
1
-
-
4
-
-
3
-
2
-
3
1
-
-
-
-
1
-
-
5
-
6
1
1
1
1
-
1
1
-
1
-
-
-
-
-
-
714027
Raman
Differential effects of hepari ...
Pedobacter heparinus
Biochem. Biophys. Res. Commun.
398
191-193
2010
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
682098
Rops
Heparan sulfate on activated g ...
Pedobacter heparinus
Nephrol. Dial. Transplant.
22
1070-1077
2007
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
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-
-
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-
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-
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-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
665613
Wei
Distinct substrate specificiti ...
Pedobacter heparinus
J. Biol. Chem.
280
15742-15748
2005
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
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-
-
-
-
-
-
-
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-
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-
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
664047
Chai
Relative susceptibilities of t ...
Pedobacter heparinus
Biochemistry
43
8590-8599
2004
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
649969
Pojasek
Histidine 295 and histidine 51 ...
Pedobacter heparinus
Biochemistry
39
4012-4019
2000
-
-
1
-
13
-
1
11
-
-
-
-
-
2
-
-
1
1
-
-
-
-
1
-
1
-
-
12
1
-
-
-
1
-
-
-
-
1
-
-
13
-
-
1
1
11
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
12
1
-
-
-
-
-
-
-
-
-
37411
Graham
Inhibition of platelet heparit ...
Homo sapiens
Biochem. Mol. Biol. Int.
37
239-246
1995
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
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-
-
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-
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-
-
-
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-
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2
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-
-
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-
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
37409
Yamada
Structural studies on the tri- ...
Pedobacter heparinus
Glycobiology
4
69-78
1994
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
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-
-
-
-
-
-
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-
-
-
-
-
-
-
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-
-
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-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
37410
Suhahara
Structural studies on the olig ...
Pedobacter heparinus
Glycobiology
4
535-544
1994
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
2
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
37412
Brickman
Heparitinase inhibition of mes ...
Pedobacter heparinus
Dev. Biol.
164
484-501
1994
-
1
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
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-
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-
-
-
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-
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-
-
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1
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1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
37407
Desai
Specificity studies on the hep ...
Pedobacter heparinus
Biochemistry
32
8140-8145
1993
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
1
-
-
-
-
-
5
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
5
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
37406
Nader H.B.; Porcionatto M.A.; Tersariol
Purification and substrate spe ...
Pedobacter heparinus
J. Biol. Chem.
265
16807-16813
1990
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
1
-
-
-
-
-
6
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
6
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
37408
Linhardt
Examination of the substrate s ...
Pedobacter heparinus
Biochemistry
29
2611-2617
1990
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
1
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
37404
Nader
Heparin sequences in the hepar ...
Pedobacter heparinus
Proc. Natl. Acad. Sci. USA
84
3565-3569
1987
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
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1
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Hovingh
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5
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