Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) | Bacteroides stercoris |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | - |
Bacteroides stercoris | |
Cu2+ | - |
Bacteroides stercoris | |
Ni2+ | - |
Bacteroides stercoris | |
Pb2+ | - |
Bacteroides stercoris | |
Zn2+ | - |
Bacteroides stercoris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
heparan sulfate | 0.0000182 g/ml | Bacteroides stercoris | |
additional information | - |
heparin | 0.00002095 g/ml | Bacteroides stercoris | |
additional information | - |
acharan sulfate | 0.00003799 g/ml | Bacteroides stercoris |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | - |
Bacteroides stercoris | |
Ca2+ | - |
Bacteroides stercoris | |
Mg2+ | - |
Bacteroides stercoris | |
Mn2+ | - |
Bacteroides stercoris | |
additional information | optimal condition: 75 mmol/l NaCl | Bacteroides stercoris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
85400 | - |
x * 85400, calculated | Bacteroides stercoris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacteroides stercoris | C0JBW5 | - |
- |
Bacteroides stercoris HJ-15 | C0JBW5 | - |
- |
Purification (Comment) | Organism |
---|---|
ion exchange chromatography | Bacteroides stercoris |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
no activity with chondroitin sulfate or hyaluronic acid | Bacteroides stercoris |
6.38 | - |
heparin | Bacteroides stercoris |
27.74 | - |
heparan sulfate | Bacteroides stercoris |
109.9 | - |
acharan sulfate | Bacteroides stercoris |
Storage Stability | Organism |
---|---|
4°C, without any additives, 15 days, retains its activity, more stable at 4°C than at -20°C, activity decreases after a few cycles of freezing and thawing | Bacteroides stercoris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acharan sulfate | uniformly repeating disaccharide structure of alpha-D-N-acetylglucosaminyl-2-O-sulfo-alpha-L-iduronic acid | Bacteroides stercoris | n 4-deoxy-2-O-sulfo-alpha-L-threo-hex-4-enopyranosyl-(1->4)-N-acetyl-D-glucosamine | - |
? | |
acharan sulfate | uniformly repeating disaccharide structure of alpha-D-N-acetylglucosaminyl-2-O-sulfo-alpha-L-iduronic acid | Bacteroides stercoris HJ-15 | n 4-deoxy-2-O-sulfo-alpha-L-threo-hex-4-enopyranosyl-(1->4)-N-acetyl-D-glucosamine | - |
? | |
heparan sulfate | - |
Bacteroides stercoris | ? | 5 products found, major product not identified | ? | |
heparan sulfate | - |
Bacteroides stercoris HJ-15 | ? | 5 products found, major product not identified | ? | |
heparin | - |
Bacteroides stercoris | n 4-deoxy-2-O-sulfo-alpha-L-threo-hex-4-enopyranosyl-(1->4)-N-acetyl-6-O-sulfo-D-glucosamine + ? | 2 minor products found | ? | |
heparin | - |
Bacteroides stercoris HJ-15 | n 4-deoxy-2-O-sulfo-alpha-L-threo-hex-4-enopyranosyl-(1->4)-N-acetyl-6-O-sulfo-D-glucosamine + ? | 2 minor products found | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 85400, calculated | Bacteroides stercoris |
Synonyms | Comment | Organism |
---|---|---|
heparin lyase II | - |
Bacteroides stercoris |
heparinase II | - |
Bacteroides stercoris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
32 | - |
- |
Bacteroides stercoris |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 37 | - |
Bacteroides stercoris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
substrate heparin | Bacteroides stercoris |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | - |
Bacteroides stercoris |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bacteroides stercoris | calculated for mature protein | - |
7.35 |