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Literature summary for 4.2.2.7 extracted from

  • Liu, D.; Shriver, Z.; Godavarti, R.; Venkataraman, G.; Sasisekharan, R.
    The calcium-binding sites of heparinase I from Flavobacterium heparinum are essential for enzymic activity (1999), J. Biol. Chem., 274, 4089-4095.
    View publication on PubMed
Search for more literature for 4.2.2.7

Cloned(Commentary)

Cloned (Comment) Organism
expression of the recombinant wild type and several mutants in Escherichia coli Pedobacter heparinus

Protein Variants

Protein Variants Comment Organism
D210A mutation does not affect the product profile compared to the wild type anzyme Pedobacter heparinus
D212A mutation does not affect the product profile compared to the wild type anzyme Pedobacter heparinus
E207A mutation does not affect the product profile compared to the wild type anzyme Pedobacter heparinus
E381A produces lower amounts of disaccharides and tetrasaccharides and a greater fraction of larger digestion fragments Pedobacter heparinus
G213A mutation does not affect the product profile compared to the wild type anzyme Pedobacter heparinus
G378A produces lower amounts of disaccharides and tetrasaccharides and a greater fraction of larger digestion fragments Pedobacter heparinus
additional information construction of several double and triple mutants of the calcium-coordinating residues Pedobacter heparinus
N375A produces lower amounts of disaccharides and tetrasaccharides and a greater fraction of larger digestion fragments Pedobacter heparinus
S377A produces lower amounts of disaccharides and tetrasaccharides and a greater fraction of larger digestion fragments Pedobacter heparinus
T216A mutation does not affect the product profile compared to the wild type anzyme Pedobacter heparinus
T373A produces lower amounts of disaccharides and tetrasaccharides and a greater fraction of larger digestion fragments Pedobacter heparinus
T382A produces lower amounts of disaccharides and tetrasaccharides and a greater fraction of larger digestion fragments Pedobacter heparinus
Y379A produces lower amounts of disaccharides and tetrasaccharides and a greater fraction of larger digestion fragments Pedobacter heparinus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 heparin several double and triple mutants of the calcium-coordinating residues Pedobacter heparinus
0.003
-
 heparin Y379A mutant, pH 7.0 30ºC Pedobacter heparinus
0.005
-
 heparin E381A mutant, pH 7.0 30ºC Pedobacter heparinus
0.007
-
 heparin G378A mutant, pH 7.0 30ºC Pedobacter heparinus
0.009
-
 heparin N375A mutant, pH 7.0 30ºC Pedobacter heparinus
0.01
-
 heparin G213A mutant, pH 7.0 30ºC Pedobacter heparinus
0.01
-
 heparin T373A mutant, pH 7.0 30ºC Pedobacter heparinus
0.015
-
 heparin T216A mutant, pH 7.0 30ºC Pedobacter heparinus
0.016
-
 heparin T382A mutant, pH 7.0 30ºC Pedobacter heparinus
0.019
-
 heparin D212A mutant, pH 7.0 30ºC Pedobacter heparinus
0.026
-
 heparin D210A mutant, pH 7.0 30ºC Pedobacter heparinus
0.027
-
 heparin S377A mutant, pH 7.0 30ºC Pedobacter heparinus
0.031
-
 heparin E207A mutant, pH 7.0 30ºC Pedobacter heparinus
0.039
-
 heparin wild type recombinant enzyme, pH 7.0 30ºC Pedobacter heparinus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ essential for activity, two calcium-binding sites with different affinity Pedobacter heparinus

Organism

Organism UniProt Comment Textmining
Pedobacter heparinus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
heparin
-
 Pedobacter heparinus unsaturated heparin disaccharide + unsaturated heparin tetrasaccharide + unsaturated heparin hexasaccharide
-
 ?

Synonyms

Synonyms Comment Organism
heparinase I
-
 Pedobacter heparinus