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Literature summary for 4.2.2.7 extracted from

  • Yang, V.C.; Linhard, R.J.; Bernstein, H.; Cooney, Ch.L.; Langer, R.
    Purification and characterization of heparinase from Flavobacterium heparinum (1985), J. Biol. Chem., 260, 1849-1857.
    View publication on PubMed

General Stability

General Stability Organism
Stability maximum: 0.15 M NaCl Pedobacter heparinus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00804
-
heparin
-
Pedobacter heparinus

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl activity maximum: 0.1 M Pedobacter heparinus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
gel filtration, SDS-PAGE, Flavobacterium heparinum Pedobacter heparinus

Organism

Organism UniProt Comment Textmining
Pedobacter heparinus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pedobacter heparinus

Storage Stability

Storage Stability Organism
Frozen or lyophilized at -20°C, protein concentration: 0.2-0.4 mg/ml, more stable in presence of 7.5% glycerol or 5% sucrose Pedobacter heparinus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
heparin
-
Pedobacter heparinus unsaturated heparin disaccharide + unsaturated heparin tetrasaccharide + unsaturated heparin hexasaccharide
-
?
Heparin monosulfate
-
Pedobacter heparinus ?
-
?
additional information not: N-acetyl heparin Pedobacter heparinus additional information
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
additional information
-
Q10: 1.45 Pedobacter heparinus
43
-
enzyme assay at Pedobacter heparinus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
rapid loss of activity above, more than 25% loss of activity after 20 min Pedobacter heparinus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Pedobacter heparinus

pH Stability

pH Stability pH Stability Maximum Comment Organism
7
-
stability maximum Pedobacter heparinus