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Literature summary for 4.2.2.5 extracted from
- Chondroitin lyase from a marine Arthrobacter sp. MAT3885 for the production of chondroitin sulfate disaccharides (2015), Mar. Biotechnol., 17, 479-492 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Arthrobacter sp. MAT3885 |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
chondroitin 6-sulfate | Km value of native enzyme is 0.058 mg/ml, of recombinant enzyme is 0.062 mg/ml, pH 7.0, 40°C | Arthrobacter sp. MAT3885 |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 80000 | - |
gel filtration | Arthrobacter sp. MAT3885 |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arthrobacter sp. MAT3885 | I6QML9 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
substrate chondroitin 6-sulfate, pH 7.0, 40°C | Arthrobacter sp. MAT3885 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chondroitin 4-sulfate | 61% of the activity with hyaluronic acid | Arthrobacter sp. MAT3885 | ? | - |
? | |
| chondroitin 6-sulfate | 86% of the activity with hyaluronic acid | Arthrobacter sp. MAT3885 | ? | - |
? | |
| hyaluronic acid | - |
Arthrobacter sp. MAT3885 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 83456, calculated from sequence | Arthrobacter sp. MAT3885 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Arthrobacter sp. MAT3885 |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
stable for 60 min | Arthrobacter sp. MAT3885 |
| 40 | - |
10 min, 65% residual activity for native enzyme, 90% residual activity for recombinant protein | Arthrobacter sp. MAT3885 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7.5 | - |
Arthrobacter sp. MAT3885 |
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Release 2023.1 (January 2023)
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