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Literature summary for 4.2.2.5 extracted from
- Insights into the structural characteristics and substrate binding analysis of chondroitin AC lyase (PsPL8A) from Pedobacter saltans (2018), Int. J. Biol. Macromol., 109, 980-991 .
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| modeling of structure. Residues N153, W105, H203, Y208, Y212, R266 and E349 are involved in catalysis | Pseudopedobacter saltans |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E349A | no residual activity | Pseudopedobacter saltans |
| H203A | no residual activity | Pseudopedobacter saltans |
| N153A | no residual activity | Pseudopedobacter saltans |
| R266A | no residual activity | Pseudopedobacter saltans |
| Y212F | no residual activity | Pseudopedobacter saltans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | presence of EDTA decreases the melting temperature | Pseudopedobacter saltans |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | presence of Ca2+ enhances the enzyme activity but does not impart any structural stability | Pseudopedobacter saltans | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudopedobacter saltans | F0S764 | - |
- |
| Pseudopedobacter saltans DSM 12145 | F0S764 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | sequence contains a N-terminal signal peptide with a cleavage site between Ala22 and Gln23 | Pseudopedobacter saltans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chondroitin 4-sulfate | - |
Pseudopedobacter saltans | ? | - |
? | |
| chondroitin 4-sulfate | - |
Pseudopedobacter saltans DSM 12145 | ? | - |
? | |
| chondroitin 6-sulfate | - |
Pseudopedobacter saltans | ? | - |
? | |
| chondroitin 6-sulfate | - |
Pseudopedobacter saltans DSM 12145 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Pedsa_3808 | - |
Pseudopedobacter saltans |
| PL8A | - |
Pseudopedobacter saltans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 52 | - |
melting temperature in presence of 5 mM EDTA | Pseudopedobacter saltans |
| 60 | - |
protein starts unfolding at 45°C and it completely melts at 60°C | Pseudopedobacter saltans |
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