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Literature summary for 4.2.2.5 extracted from

  • Shim, K.W.; Kim, D.H.
    Cloning and expression of chondroitinase AC from Bacteroides stercoris HJ-15 (2008), Protein Expr. Purif., 58, 222-228.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Bacteroides stercoris

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
Bacteroides stercoris
heparin
-
Bacteroides stercoris
Ni2+
-
Bacteroides stercoris
Zn2+
-
Bacteroides stercoris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.155
-
chondroitin sulfate C native protein Bacteroides stercoris
0.167
-
chondroitin sulfate C recombinant protein Bacteroides stercoris
0.234
-
hyaluronic acid recombinant protein Bacteroides stercoris
0.259
-
hyaluronic acid native protein Bacteroides stercoris
0.38
-
chondroitin sulfate A native protein Bacteroides stercoris
0.42
-
chondroitin sulfate A recombinant protein Bacteroides stercoris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
SDS-PAGE Bacteroides stercoris

Organism

Organism UniProt Comment Textmining
Bacteroides stercoris A9Y5J0
-
-

Purification (Commentary)

Purification (Comment) Organism
using His bind column chromatography Bacteroides stercoris

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.75
-
-
Bacteroides stercoris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chondroitin sulfate A relative activity: 100% (recombinant protein), 100% (native protein) Bacteroides stercoris ?
-
?
chondroitin sulfate C relative activity: 42% (recombinant protein), 46% (native protein) Bacteroides stercoris ?
-
?
hyaluronic acid relative activity: 54% (recombinant protein), 67% (native protein) Bacteroides stercoris ?
-
?
additional information no activity with dermatan sulfate or heparin as substrate Bacteroides stercoris ?
-
?

Synonyms

Synonyms Comment Organism
chondroitinase AC
-
Bacteroides stercoris