Crystallization (Comment) | Organism |
---|---|
native enzyme alone and in complex with substrates chondroitin 4-sulfate tetrasaccharide, and hyaluronan tetrasaccharide, hanging drop vapour diffusion method, 0.002 ml protein solution: 10 mg/ml protein, + 0.002 ml reservoir solution: 23% w/v PEG 8000, 0.1 M sodium phosphate, pH 6.4, 0.4 M ammonium acetate, 10% v/v glycerol, suspended over 1 ml reservoir solution, a few days, larger crystals by macroseeding, X-ray diffraction structure determination and analysis of the enzyme-substrate complexes at 1.25-1.9 A high resolution | Paenarthrobacter aurescens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
79500 | 79840 | mass spectrometry | Paenarthrobacter aurescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenarthrobacter aurescens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Paenarthrobacter aurescens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
[1,3-beta-D-glucuronic acid-1,4-beta-D-hexosylamine]n = n-1 4-deoxy-beta-D-gluc-4-enuronosyl-1,4-beta-D-hexosylamine + [1,3-beta-D-glucuronic acid-1,4-beta-D-hexosylamine] | acts on chondroitin 4-sulfate and chondroitin 6-sulfate, but less well on hyaluronate, lytic mechanism with Tyr242 acting as a general base that abstracts the proton from the C5 position of glucuronic acid while Asn183 and His233 neutralize the charge on the glucuronate acidic group, substrate binding site | Paenarthrobacter aurescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chondroitin sulfate A | i.e. chondroitin 4-sulfate | Paenarthrobacter aurescens | ? | - |
? | |
chondroitin sulfate C | i.e. chondroitin 6-sulfate | Paenarthrobacter aurescens | ? | - |
? | |
hyaluronan | - |
Paenarthrobacter aurescens | ? | - |
? | |
additional information | substrate specificity, no activity with dermatan sulfate | Paenarthrobacter aurescens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
chondroitin lyase AC | - |
Paenarthrobacter aurescens |