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Literature summary for 4.2.2.5 extracted from
- Isolation and expression in Escherichia coli of cslA and cslB, genes coding for the chondroitin sulfate-degrading enzymes chondroitinase AC and chondroitinase B, respectively, from Flavobacterium heparinum (2000), Appl. Environ. Microbiol., 66, 29-35.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| chondroitin 4-sulfate | 2.5fold induction of enzyme expression | Pedobacter heparinus |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene cslA, DNA sequence determination and analysis, chromosomal localization, expression in Escherichia coli | Pedobacter heparinus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| periplasm | - |
Pedobacter heparinus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
x * 70000, recombinant enzyme, SDS-PAGE | Pedobacter heparinus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pedobacter heparinus | Q59288 | gene cslA | - |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| [1,3-beta-D-glucuronic acid-1,4-beta-D-hexosylamine]n = n-1 4-deoxy-beta-D-gluc-4-enuronosyl-1,4-beta-D-hexosylamine + [1,3-beta-D-glucuronic acid-1,4-beta-D-hexosylamine] | acts on chondroitin 4-sulfate and chondroitin 6-sulfate, but less well on hyaluronate | Pedobacter heparinus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Pedobacter heparinus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chondroitin sulfate A | i.e. chondroitin 4-sulfate | Pedobacter heparinus | ? | - |
? | |
| chondroitin sulfate C | i.e. chondroitin 6-sulfate | Pedobacter heparinus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 70000, recombinant enzyme, SDS-PAGE | Pedobacter heparinus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ChnAC | - |
Pedobacter heparinus |
| chondroitinase AC | - |
Pedobacter heparinus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Pedobacter heparinus |
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