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Literature summary for 4.2.2.5 extracted from

  • Gu, K.; Linhardt, R.L.; Laliberte, M.; Gu, K.; Zimmermann, J.
    Purification, characterization and specificity of chondroitin lyases and glycuronidase from Flavobacterium heparinum (1995), Biochem. J., 312, 569-577.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.2.5

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0093
-
 chondroitin 6-sulfate
-
 Pedobacter heparinus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
74000
-
 x * 74000, SDS-PAGE Pedobacter heparinus

Organism

Organism UniProt Comment Textmining
Pedobacter heparinus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pedobacter heparinus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
68
-
 chondroitin 4-sulfate Pedobacter heparinus
111
-
 chondroitin 6-sulfate Pedobacter heparinus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chondroitin 4-sulfate
-
 Pedobacter heparinus 2-acetamido-2-deoxy-3-O-(beta-D-gluco-4-enepyranosyluronic acid)-4-O-sulfo-D-galactose
-
 ?
chondroitin 6-sulfate
-
 Pedobacter heparinus ?
-
 ?
dermatan sulfate
-
 Pedobacter heparinus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 74000, SDS-PAGE Pedobacter heparinus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Pedobacter heparinus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 6 h, 50% loss of activity Pedobacter heparinus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7.5
-
 Pedobacter heparinus