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Literature summary for 4.2.2.3 extracted from

  • Manns, D.; Nyffenegger, C.; Saake, B.; Meyer, A.
    Impact of different alginate lyases on combined cellulase-lyase saccharification of brown seaweed (2016), RSC Adv., 6, 45392-45401 .
No PubMed abstract available
Search for more literature for 4.2.2.3

Application

Application Comment Organism
degradation enzymatic treatment for 24 hours is sufficient to release all potential glucose from the glucan rich brown seaweed Laminaria digitata Sphingomonas sp. A1
degradation enzymatic treatment for 24 hours is sufficient to release all potential glucose from the glucan rich brown seaweed Laminaria digitata Flavobacterium sp. S20

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Sphingomonas sp. A1
expression in Escherichia coli Flavobacterium sp. S20

Organism

Organism UniProt Comment Textmining
Flavobacterium sp. S20 G9CHX6
-
-
Sphingomonas sp. A1 Q75WP3
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alginate
-
 Sphingomonas sp. A1 unsaturated algino-oligosaccharides
-
 ?
alginate
-
 Flavobacterium sp. S20 unsaturated algino-oligosaccharides
-
 ?
additional information enzyme prefers poly-D-mannuronate blocks over poly-L-guluronate blocks Flavobacterium sp. S20 ?
-
 ?
additional information enzyme prefers poly-L-guluronate blocks over poly-D-mannuronate blocks Sphingomonas sp. A1 ?
-
 ?
poly(alpha-(1,4)-L-guluronate) reaction of EC 4.2.2.11 Sphingomonas sp. A1 4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1,4)-alpha-oligo(alpha-(1,4)-L-guluronate)
-
 ?
poly(alpha-1,4-L-guluronate) reaction of EC 4.2.2.11 Flavobacterium sp. S20 4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1,4)-alpha-oligo(alpha-(1,4)-L-guluronate)
-
 ?
poly(beta-(1,4)-D-mannuronate)
-
 Sphingomonas sp. A1 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-(1,4)-beta-oligo(beta-(1,4)-D-mannuronate)
-
 ?
poly(beta-1,4-D-mannuronate)
-
 Flavobacterium sp. S20 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-(1,4)-beta-oligo(beta-(1,4)-D-mannuronate)
-
 ?

Synonyms

Synonyms Comment Organism
Alg2A
-
 Flavobacterium sp. S20

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 stable up to Sphingomonas sp. A1

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 7
-
 Sphingomonas sp. A1
7.5
-
-
 Flavobacterium sp. S20