Any feedback?
Literature summary for 4.2.2.3 extracted from
- Cloning and characterization of a new pH-stable alginate lyase with high salt tolerance from marine Vibrio sp. NJ-04 (2018), Int. J. Biol. Macromol., 115, 1063-1070 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Vibrio sp. NJ-04 |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | 1 mM, 70% of initial activity | Vibrio sp. NJ-04 |  |
| Zn2+ | 1 mM, 57% of initial activity | Vibrio sp. NJ-04 | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.24 | - |
poly(alpha-(1,4)-L-guluronate) | pH 8.0, 40°C | Vibrio sp. NJ-04 | |
| 0.49 | - |
alginate | pH 8.0, 40°C | Vibrio sp. NJ-04 | |
| 0.86 | - |
poly(beta-(1,4)-D-mannuronate) | pH 8.0, 40°C | Vibrio sp. NJ-04 | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 1 mM, 125% of initial activity | Vibrio sp. NJ-04 | |
| Cu2+ | 1 mM, 18% of initial activity | Vibrio sp. NJ-04 | |
| K+ | 1 mM, 136% of initial activity | Vibrio sp. NJ-04 | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vibrio sp. NJ-04 | A0A1Z2QRD5 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2043 | - |
substrate poly(beta-(1,4)-D-mannuronate), pH 7.0, 40°C | Vibrio sp. NJ-04 |
| 2416 | - |
substrate alginate, pH 7.0, 40°C | Vibrio sp. NJ-04 |
| 3359 | - |
substrate poly(alpha-(1,4)-L-guluronate), pH 7.0, 40°C | Vibrio sp. NJ-04 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alginate | - |
Vibrio sp. NJ-04 | unsaturated algino-oligosaccharides | - |
? | |
| poly(alpha-(1,4)-L-guluronate) | reaction of EC 4.2.2.11 | Vibrio sp. NJ-04 | 4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1,4)-alpha-oligo(alpha-(1,4)-L-guluronate) | - |
? | |
| poly(beta-(1,4)-D-mannuronate) | - |
Vibrio sp. NJ-04 | 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-(1,4)-beta-oligo(beta-(1,4)-D-mannuronate) | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 50190, calculated from sequence, x * 50000, SDS-PAGE | Vibrio sp. NJ-04 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Vibrio sp. NJ-04 |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 29 | - |
poly(alpha-(1,4)-L-guluronate) | pH 8.0, 40°C | Vibrio sp. NJ-04 | |
| 59 | - |
alginate | pH 8.0, 40°C | Vibrio sp. NJ-04 | |
| 77 | - |
poly(beta-(1,4)-D-mannuronate) | pH 8.0, 40°C | Vibrio sp. NJ-04 | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Vibrio sp. NJ-04 |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | more than 80% of maximum activity | Vibrio sp. NJ-04 |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 89.5 | - |
poly(beta-(1,4)-D-mannuronate) | pH 8.0, 40°C | Vibrio sp. NJ-04 | |
| 120.4 | - |
alginate | pH 8.0, 40°C | Vibrio sp. NJ-04 | |
| 120.8 | - |
poly(alpha-(1,4)-L-guluronate) | pH 8.0, 40°C | Vibrio sp. NJ-04 | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
