Literature summary for 4.2.2.3 extracted from

Yu, Z.; Zhu, B.; Wang, W.; Tan, H.; Yin, H.
Characterization of a new oligoalginate lyase from marine bacterium Vibrio sp. (2018), Int. J. Biol. Macromol., 112, 937-942 .

Search for more literature for 4.2.2.3

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escheriochia coli	Vibrio sp. W13

Inhibitors

Inhibitors	Comment	Organism	Structure
Ca2+	1 mM, 18% of initial activity	Vibrio sp. W13
Co2+	1 mM, 71% of initial activity	Vibrio sp. W13
Cu2+	1 mM, 71% of initial activity	Vibrio sp. W13
EDTA	1 mM, 46% of initial activity	Vibrio sp. W13
Mn2+	1 mM, 75% of initial activity	Vibrio sp. W13
SDS	1 mM, 23% of initial activity	Vibrio sp. W13

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Na+	50 mM, 124% of initial activity	Vibrio sp. W13

Organism

Organism	UniProt	Comment	Textmining
Vibrio sp. W13	A0A0F7KQI4	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
7.43	-	substrate poly(alpha-(1,4)-L-guluronate), pH 7.0, 30°C	Vibrio sp. W13
12.31	-	substrate poly(beta-(1,4)-D-mannuronate), pH 7.0, 30°C	Vibrio sp. W13
21.14	-	substrate alginate, pH 7.0, 30°C	Vibrio sp. W13

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
alginate	-	Vibrio sp. W13	unsaturated algino-oligosaccharides	-	?
poly(alpha-(1,4)-L-guluronate)	reaction of EC 4.2.2.11	Vibrio sp. W13	4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1,4)-alpha-oligo(alpha-(1,4)-L-guluronate)	-	?
poly(beta-(1,4)-D-mannuronate)	-	Vibrio sp. W13	4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-(1,4)-beta-oligo(beta-(1,4)-D-mannuronate)	-	?

Subunits

Subunits	Comment	Organism
?	x * 81020, calculated from sequence, x * 81000, SDS-PAGE	Vibrio sp. W13

Synonyms

Synonyms	Comment	Organism
Oal17A	-	Vibrio sp. W13

pI Value

Organism	Comment	pI Value Maximum	pI Value
Vibrio sp. W13	calculated from sequence	-	5

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Release 2023.1 (January 2023)