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Literature summary for 4.2.2.3 extracted from

  • Chen, X.; Dong, S.; Xu, F.; Dong, F.; Li, P.; Zhang, X.; Zhou, B.; Zhang, Y.; Xie, B.
    Characterization of a new cold-adapted and salt-activated polysaccharide lyase family 7 alginate lyase from Pseudoalteromonas sp. SM0524 (2016), Front. Microbiol., 7, 1120 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.2.3

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudoalteromonas sp. SM0524

Inhibitors

Inhibitors Comment Organism Structure
Ni2+ 2 mM, 46% of initial activity Pseudoalteromonas sp. SM0524

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 2 mM, 130% of initial activity Pseudoalteromonas sp. SM0524
Co2+ 2 mM, 148% of initial activity Pseudoalteromonas sp. SM0524
Cu2+ 2 mM, 133% of initial activity Pseudoalteromonas sp. SM0524
Mg2+ 2 mM, 146% of initial activity Pseudoalteromonas sp. SM0524
Mn2+ 2 mM, 125% of initial activity Pseudoalteromonas sp. SM0524
Na+ activity in 0.5-1.2 M NaCl is sixfold higher than that in absence of NaCl. The Km value in 0.5 M NaCl decreases more than 20fold compared to that in absence of NaCl Pseudoalteromonas sp. SM0524

Organism

Organism UniProt Comment Textmining
Pseudoalteromonas sp. SM0524 B2LME8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alginate
-
 Pseudoalteromonas sp. SM0524 unsaturated algino-oligosaccharides
-
 ?
poly(alpha-(1,4)-L-guluronate) reaction of EC 4.2.2.11, about 15% of the activity with poly(beta-(1,4)-D-mannuronate) Pseudoalteromonas sp. SM0524 4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1,4)-alpha-oligo(alpha-(1,4)-L-guluronate)
-
 ?
poly(beta-(1,4)-D-mannuronate)
-
 Pseudoalteromonas sp. SM0524 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-(1,4)-beta-oligo(beta-(1,4)-D-mannuronate)
-
 ?

Subunits

Subunits Comment Organism
? x * 37100, calculated from sequence, x * 40000, SDS-PAGE Pseudoalteromonas sp. SM0524

Synonyms

Synonyms Comment Organism
AlyPM
-
 Pseudoalteromonas sp. SM0524

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Pseudoalteromonas sp. SM0524

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5
-
 19% of maximum activity Pseudoalteromonas sp. SM0524

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 unstable above Pseudoalteromonas sp. SM0524
37
-
 melting temperature Pseudoalteromonas sp. SM0524

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Pseudoalteromonas sp. SM0524

pH Range

pH Minimum pH Maximum Comment Organism
7 9.5 more than 70% of maximum activity Pseudoalteromonas sp. SM0524