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Literature summary for 4.2.2.3 extracted from

  • Zhu, B.; Ni, F.; Sun, Y.; Yao, Z.
    Expression and characterization of a new heat-stable endo-type alginate lyase from deep-sea bacterium Flammeovirga sp. NJ-04 (2017), Extremophiles, 21, 1027-1036 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Flammeovirga sp. NJ-04

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.48
-
alginate pH 7.0, 50°C Flammeovirga sp. NJ-04
0.94
-
poly(alpha-(1,4)-L-guluronate) pH 7.0, 50°C Flammeovirga sp. NJ-04
1.42
-
poly(beta-(1,4)-D-mannuronate) pH 7.0, 50°C Flammeovirga sp. NJ-04

Organism

Organism UniProt Comment Textmining
Flammeovirga sp. NJ-04 A0A1Z2QRE2
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme purified on Ni-NTA sepharose Flammeovirga sp. NJ-04

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1487.6
-
substrate poly(beta-(1,4)-D-mannuronate), pH 7.0, 50°C Flammeovirga sp. NJ-04
2604.9
-
substrate poly(alpha-(1,4)-L-guluronate), pH 7.0, 50°C Flammeovirga sp. NJ-04
3343.7
-
substrate alginate, pH 7.0, 50°C Flammeovirga sp. NJ-04

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alginate
-
Flammeovirga sp. NJ-04 unsaturated algino-oligosaccharides
-
?
additional information enzyme shows high activities toward both poly(beta-D-mannuronate) and poly(alpha-L-guluronate), reactions of EC 4.2.2.3 and 4.2.2.11, respectively Flammeovirga sp. NJ-04 ?
-
?
poly(alpha-(1,4)-L-guluronate) reaction of EC 4.2.2.11 Flammeovirga sp. NJ-04 4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1,4)-alpha-oligo(alpha-(1,4)-L-guluronate)
-
?
poly(beta-(1,4)-D-mannuronate)
-
Flammeovirga sp. NJ-04 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-(1,4)-beta-oligo(beta-(1,4)-D-mannuronate)
-
?

Subunits

Subunits Comment Organism
? x * 59000, calculated from sequence, without signal peptide Flammeovirga sp. NJ-04

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Flammeovirga sp. NJ-04

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
30 min, more than 80% residual activity Flammeovirga sp. NJ-04

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.52
-
poly(beta-(1,4)-D-mannuronate) pH 7.0, 50°C Flammeovirga sp. NJ-04
3.37
-
poly(alpha-(1,4)-L-guluronate) pH 7.0, 50°C Flammeovirga sp. NJ-04
3.86
-
alginate pH 7.0, 50°C Flammeovirga sp. NJ-04

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Flammeovirga sp. NJ-04

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9 24 h, more than 50% residual activity Flammeovirga sp. NJ-04

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.78
-
poly(beta-(1,4)-D-mannuronate) pH 7.0, 50°C Flammeovirga sp. NJ-04
3.59
-
poly(alpha-(1,4)-L-guluronate) pH 7.0, 50°C Flammeovirga sp. NJ-04
8.03
-
alginate pH 7.0, 50°C Flammeovirga sp. NJ-04