Cloned (Comment) | Organism |
---|---|
recombinant expression of N-terminally GST-tagged enzyme in Escherichia coli strains BL21(DE3), C41(DE3) and C43(DE3) | Pseudomonas alginovora |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required | Pseudomonas alginovora |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas alginovora | - |
- |
- |
Pseudomonas alginovora X017 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | hydrolysis of the crude alginate since beta-elimination on beta-D-mannuronosyluronate or on alpha-L-gulupyranosyluronate produces, in both cases, the nonreducing end 4-deoxy-L-erythro-hex-4-enepyranosyluronate. Products are mainly trisaccharides, followed by tetrasaccharides, disaccharides, and pentasaccharides in descending order, gel filtration, and mass and NMR spectrometric analyses, overview | Pseudomonas alginovora | ? | - |
? | |
additional information | hydrolysis of the crude alginate since beta-elimination on beta-D-mannuronosyluronate or on alpha-L-gulupyranosyluronate produces, in both cases, the nonreducing end 4-deoxy-L-erythro-hex-4-enepyranosyluronate. Products are mainly trisaccharides, followed by tetrasaccharides, disaccharides, and pentasaccharides in descending order, gel filtration, and mass and NMR spectrometric analyses, overview | Pseudomonas alginovora X017 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alginate lyase | - |
Pseudomonas alginovora |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Pseudomonas alginovora |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Pseudomonas alginovora |