Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.2.3 extracted from

  • Park, H.; Kam, N.; Lee, E.; Kim, H.
    Cloning and characterization of a novel oligoalginate lyase from a newly isolated bacterium Sphingomonas sp. MJ-3 (2012), Mar. Biotechnol., 14, 189-202.
    View publication on PubMed

Application

Application Comment Organism
synthesis the recombinant MJ-3 alginate lyase can be used as a biocatalyst for saccharification of alginate since it can efficiently degrade poly-M block, poly-G block, poly-MG block, alginate oligosaccharides, and alginate into alginate monosaccharides Sphingomonas sp.

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Sphingomonas sp.
expression in Escherichia coli Sphingomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
78000
-
x * 79900, including signal peptide, x * 78000, without signal peptide, calculated. x * 80000, SDS-PAGE Sphingomonas sp.
79600
-
x * 79600, recombinant His6-tagged enzyme, SDS-PAGE Sphingomonas sp.
79900
-
x * 79900, including signal peptide, x * 78000, without signal peptide, calculated. x * 80000, SDS-PAGE Sphingomonas sp.
80000
-
x * 79900, including signal peptide, x * 78000, without signal peptide, calculated. x * 80000, SDS-PAGE Sphingomonas sp.

Organism

Organism UniProt Comment Textmining
Sphingomonas sp. G1EH67
-
-
Sphingomonas sp. G1EH67 isolated from fermented brown seaweed with salted and fermented anchovy, sea mustard, Undaria pinnatifida
-
Sphingomonas sp. MJ-3 G1EH67
-
-
Sphingomonas sp. MJ-3 G1EH67 isolated from fermented brown seaweed with salted and fermented anchovy, sea mustard, Undaria pinnatifida
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Sphingomonas sp.
recombinant protein Sphingomonas sp.

Source Tissue

Source Tissue Comment Organism Textmining
additional information the organism is able to grow on alginate as the sole carbon source Sphingomonas sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-mannuronyl linkage in alginate
-
Sphingomonas sp. ? final degradation products are alginate monosaccharides ?
beta-D-mannuronyl linkage in alginate
-
Sphingomonas sp. MJ-3 ? final degradation products are alginate monosaccharides ?
additional information substrate specificity of recombinant oligoalginate lyase, overview. Analysis of the final degradation products, overview Sphingomonas sp. ?
-
?
additional information substrate specificity of recombinant oligoalginate lyase, overview. Analysis of the final degradation products, overview Sphingomonas sp. MJ-3 ?
-
?
poly(beta-(1->4)-D-mannuronic acid) 190% of the activity with alginate Sphingomonas sp. ?
-
?
poly(beta-(1->4)-D-mannuronic acid) 190% of the activity with alginate Sphingomonas sp. MJ-3 ?
-
?
poly(beta-(1->4)-D-mannuronic acid/alpha-(1->4)-L-guluronic acid) alternating structure of alpha-L-guluronic acid and beta-D-mannuronic acid. 120% of the activity with alginate Sphingomonas sp. ?
-
?
poly(beta-(1->4)-D-mannuronic acid/alpha-(1->4)-L-guluronic acid) alternating structure of alpha-L-guluronic acid and beta-D-mannuronic acid. 120% of the activity with alginate Sphingomonas sp. MJ-3 ?
-
?

Subunits

Subunits Comment Organism
? x * 79600, recombinant His6-tagged enzyme, SDS-PAGE Sphingomonas sp.
? x * 79900, including signal peptide, x * 78000, without signal peptide, calculated. x * 80000, SDS-PAGE Sphingomonas sp.

Synonyms

Synonyms Comment Organism
MJ-3 alginate lyase
-
Sphingomonas sp.
oligoalginate lyase
-
Sphingomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Sphingomonas sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 80 activity range Sphingomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Sphingomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
5 9 activity range Sphingomonas sp.