Application | Comment | Organism |
---|---|---|
synthesis | the recombinant MJ-3 alginate lyase can be used as a biocatalyst for saccharification of alginate since it can efficiently degrade poly-M block, poly-G block, poly-MG block, alginate oligosaccharides, and alginate into alginate monosaccharides | Sphingomonas sp. |
Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Sphingomonas sp. |
expression in Escherichia coli | Sphingomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
78000 | - |
x * 79900, including signal peptide, x * 78000, without signal peptide, calculated. x * 80000, SDS-PAGE | Sphingomonas sp. |
79600 | - |
x * 79600, recombinant His6-tagged enzyme, SDS-PAGE | Sphingomonas sp. |
79900 | - |
x * 79900, including signal peptide, x * 78000, without signal peptide, calculated. x * 80000, SDS-PAGE | Sphingomonas sp. |
80000 | - |
x * 79900, including signal peptide, x * 78000, without signal peptide, calculated. x * 80000, SDS-PAGE | Sphingomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingomonas sp. | G1EH67 | - |
- |
Sphingomonas sp. | G1EH67 | isolated from fermented brown seaweed with salted and fermented anchovy, sea mustard, Undaria pinnatifida | - |
Sphingomonas sp. MJ-3 | G1EH67 | - |
- |
Sphingomonas sp. MJ-3 | G1EH67 | isolated from fermented brown seaweed with salted and fermented anchovy, sea mustard, Undaria pinnatifida | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Sphingomonas sp. |
recombinant protein | Sphingomonas sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | the organism is able to grow on alginate as the sole carbon source | Sphingomonas sp. | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-mannuronyl linkage in alginate | - |
Sphingomonas sp. | ? | final degradation products are alginate monosaccharides | ? | |
beta-D-mannuronyl linkage in alginate | - |
Sphingomonas sp. MJ-3 | ? | final degradation products are alginate monosaccharides | ? | |
additional information | substrate specificity of recombinant oligoalginate lyase, overview. Analysis of the final degradation products, overview | Sphingomonas sp. | ? | - |
? | |
additional information | substrate specificity of recombinant oligoalginate lyase, overview. Analysis of the final degradation products, overview | Sphingomonas sp. MJ-3 | ? | - |
? | |
poly(beta-(1->4)-D-mannuronic acid) | 190% of the activity with alginate | Sphingomonas sp. | ? | - |
? | |
poly(beta-(1->4)-D-mannuronic acid) | 190% of the activity with alginate | Sphingomonas sp. MJ-3 | ? | - |
? | |
poly(beta-(1->4)-D-mannuronic acid/alpha-(1->4)-L-guluronic acid) | alternating structure of alpha-L-guluronic acid and beta-D-mannuronic acid. 120% of the activity with alginate | Sphingomonas sp. | ? | - |
? | |
poly(beta-(1->4)-D-mannuronic acid/alpha-(1->4)-L-guluronic acid) | alternating structure of alpha-L-guluronic acid and beta-D-mannuronic acid. 120% of the activity with alginate | Sphingomonas sp. MJ-3 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 79600, recombinant His6-tagged enzyme, SDS-PAGE | Sphingomonas sp. |
? | x * 79900, including signal peptide, x * 78000, without signal peptide, calculated. x * 80000, SDS-PAGE | Sphingomonas sp. |
Synonyms | Comment | Organism |
---|---|---|
MJ-3 alginate lyase | - |
Sphingomonas sp. |
oligoalginate lyase | - |
Sphingomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Sphingomonas sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 80 | activity range | Sphingomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Sphingomonas sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | activity range | Sphingomonas sp. |