Any feedback?
Literature summary for 4.2.2.3 extracted from
- Heterologous expression of an alginate lyase from Streptomyces sp. ALG-5 in Escherichia coli and its use for preparation of the magnetic nanoparticle-immobilized enzymes (2011), Bioprocess Biosyst. Eng., 34, 113-119.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) at 37°C and at 15°C. The recombinant alginate lyase expressed with pColdI at 15°C exhibits the highest alginate-degrading activity, method evaluation and optimization | Streptomyces sp. |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | recombinant alginate lyase is efficiently immobilized onto two types of magnetic nanoparticles, superparamagnetic iron oxide nanoparticle, and hybrid magnetic silica nanoparticle, based on the affinity between His-tag and Ni2+ displayed on the surfaces of nanoparticles. An alginate oligosaccharide mixture consisting of dimer and trimer is synthesized by the immobilized alginate lyase. The immobilized enzymes can be re-used repeatedly more than 10 times after magnetic separation, overview | Streptomyces sp. |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates | Streptomyces sp. |  |
| Co2+ | activates | Streptomyces sp. | |
| Mg2+ | activates | Streptomyces sp. | |
| Mn2+ | activates | Streptomyces sp. | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27500 | - |
x * 27500, recombinant His-tagged enzyme, SDS-PAGE | Streptomyces sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces sp. | - |
- |
- |
| Streptomyces sp. ALG-5 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Streptomyces sp. |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 27500, recombinant His-tagged enzyme, SDS-PAGE | Streptomyces sp. |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alginate lyase | - |
Streptomyces sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Streptomyces sp. |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 80 | - |
Streptomyces sp. |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
activity is stable up to 40°C for 30 min reaction, and then sharply decreases with further increase in temperature | Streptomyces sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | - |
Streptomyces sp. |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.6 | 11 | - |
Streptomyces sp. |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the marine alginate lyase from Streptomyces sp. ALG-5 specifically degrades poly-G block of alginate | Streptomyces sp. |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
