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Literature summary for 4.2.2.3 extracted from
- Three alginate lyases from marine bacterium Pseudomonas fluorescens HZJ216: Purification and characterization (2011), Appl. Biochem. Biotechnol., 164, 305-317.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | 50 mM, isoform A 89%, isoform B 44%, isoform C 42% of initial activity | Pseudomonas fluorescens |  |
| Co2+ | 50 mM, isoform A 67%, isoform B 66%, isoform C 29% of initial activity | Pseudomonas fluorescens | |
| Fe2+ | 50 mM, no residual activity; complete inhibition of all isozymes at 50 mM | Pseudomonas fluorescens | |
| Fe3+ | - |
Pseudomonas fluorescens | |
| Zn2+ | 50 mM, isoform A 10%, isoform B 14%, isoform C 12% of initial activity | Pseudomonas fluorescens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates | Pseudomonas fluorescens | |
| Ca2+ | 50 mM, isoform A 117%, isoform B 131% of initial activity | Pseudomonas fluorescens | |
| Cu2+ | activates | Pseudomonas fluorescens | |
| K+ | activates | Pseudomonas fluorescens | |
| K+ | 50 mM, isoform A 132%, isoform B 110% of initial activity | Pseudomonas fluorescens | |
| Mg2+ | activates | Pseudomonas fluorescens | |
| Mg2+ | 50 mM, isoform A 116%, isoform B 121%, isoform C 114% of initial activity | Pseudomonas fluorescens | |
| additional information | no effect by Li+ at 50 mM | Pseudomonas fluorescens | |
| Na+ | is essential for the activity of all the three lyases | Pseudomonas fluorescens | |
| NaCl | activates at 1.5% | Pseudomonas fluorescens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23000 | - |
x * 60250, alginate lyase A, SDS-PAGE, x * 36000, alginate lyase B, SDS-PAGE, x * 23000, alginate lyase C, SDS-PAGE | Pseudomonas fluorescens |
| 23000 | - |
x * 60250, isoform A, x * 36000, isoform B, x * 23000, isoform C, SDS-PAGE | Pseudomonas fluorescens |
| 36000 | - |
x * 60250, alginate lyase A, SDS-PAGE, x * 36000, alginate lyase B, SDS-PAGE, x * 23000, alginate lyase C, SDS-PAGE | Pseudomonas fluorescens |
| 36000 | - |
x * 60250, isoform A, x * 36000, isoform B, x * 23000, isoform C, SDS-PAGE | Pseudomonas fluorescens |
| 60250 | - |
x * 60250, alginate lyase A, SDS-PAGE, x * 36000, alginate lyase B, SDS-PAGE, x * 23000, alginate lyase C, SDS-PAGE | Pseudomonas fluorescens |
| 60250 | - |
x * 60250, isoform A, x * 36000, isoform B, x * 23000, isoform C, SDS-PAGE | Pseudomonas fluorescens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Pseudomonas fluorescens | alginate lyase A degrades M and G blocks, and the enzyme activity acting on M block is much more than that of G block, while for alginate lyase B, the enzyme activities on M block are slightly higher than that of G block | ? | - |
? | |
| additional information | Pseudomonas fluorescens HZJ216 | alginate lyase A degrades M and G blocks, and the enzyme activity acting on M block is much more than that of G block, while for alginate lyase B, the enzyme activities on M block are slightly higher than that of G block | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas fluorescens | - |
- |
- |
| Pseudomonas fluorescens | - |
isolated from brown seaweed in the Yellow Sea of China | - |
| Pseudomonas fluorescens HZJ216 | - |
- |
- |
| Pseudomonas fluorescens HZJ216 | - |
isolated from brown seaweed in the Yellow Sea of China | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native alginate lyases, by acetone precipitation, anion exchange chromatography, and gel filtration | Pseudomonas fluorescens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 21.77 | - |
alginate lyase C, substrate with M blocks, pH 7.0, 30°C | Pseudomonas fluorescens |
| 129 | - |
alginate lyase C, substrate with G blocks, pH 7.0, 30°C | Pseudomonas fluorescens |
| 330 | - |
alginate lyase B, substrate with G blocks, pH 7.0, 30°C | Pseudomonas fluorescens |
| 382 | - |
alginate lyase B, substrate with M blocks, pH 7.0, 30°C | Pseudomonas fluorescens |
| 382 | - |
isoform B, substrate poly(beta-D-mannuronate), pH 7.0, 30°C | Pseudomonas fluorescens |
| 390 | - |
alginate lyase A, substrate with G blocks, pH 7.0, 30°C | Pseudomonas fluorescens |
| 467 | - |
isoform B, substrate sodium alginate, pH 7.0, 30°C | Pseudomonas fluorescens |
| 651 | - |
alginate lyase A, substrate with M blocks, pH 7.0, 30°C | Pseudomonas fluorescens |
| 651 | - |
isoform A, substrate poly(beta-D-mannuronate), pH 7.0, 30°C | Pseudomonas fluorescens |
| 653 | - |
isoform A, substrate sodium alginate, pH 7.0, 30°C | Pseudomonas fluorescens |
| 1123 | - |
extracellular enzyme, pH 7.0, 30°C | Pseudomonas fluorescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-L-mannuronyl linkage in alginate | alginate lyases A and B have the activities for both M and G blocks. For isoform A, the enzyme activity acting on M block is much more than that of G block, for alginate lyase B, the enzyme activity on M block is slightly higher than that on G block and there is no obvious substrate specificity difference between them | Pseudomonas fluorescens | ? | - |
? | |
| alpha-L-mannuronyl linkage in alginate | alginate lyases A and B have the activities for both M and G blocks. For isoform A, the enzyme activity acting on M block is much more than that of G block, for alginate lyase B, the enzyme activity on M block is slightly higher than that on G block and there is no obvious substrate specificity difference between them | Pseudomonas fluorescens HZJ216 | ? | - |
? | |
| additional information | alginate lyase A degrades M and G blocks, and the enzyme activity acting on M block is much more than that of G block, while for alginate lyase B, the enzyme activities on M block are slightly higher than that of G block | Pseudomonas fluorescens | ? | - |
? | |
| additional information | alginate lyase A degrades M and G blocks, and the enzyme activity acting on M block is much more than that of G block, while for alginate lyase B, the enzyme activities on M block are slightly higher than that of G block | Pseudomonas fluorescens HZJ216 | ? | - |
? | |
| poly(beta-(1->4)-D-mannuronate) | - |
Pseudomonas fluorescens | ? | - |
? | |
| poly(beta-(1->4)-D-mannuronate) | - |
Pseudomonas fluorescens HZJ216 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 60250, alginate lyase A, SDS-PAGE, x * 36000, alginate lyase B, SDS-PAGE, x * 23000, alginate lyase C, SDS-PAGE | Pseudomonas fluorescens |
| ? | x * 60250, isoform A, x * 36000, isoform B, x * 23000, isoform C, SDS-PAGE | Pseudomonas fluorescens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alginate lyase A | - |
Pseudomonas fluorescens |
| alginate lyase B | - |
Pseudomonas fluorescens |
| alginate lyase C | - |
Pseudomonas fluorescens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 3.5 | - |
- |
Pseudomonas fluorescens |
| 35 | - |
- |
Pseudomonas fluorescens |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
isoform C, 54% of maximum activity | Pseudomonas fluorescens |
| 25 | 60 | activity range, the lyases possess more than 60% activity in the range of 25°C to 40°C except alginate lyase C at 25°C which is 53.89% activity, but the activities decrease above 40°C | Pseudomonas fluorescens |
| 25 | 40 | isoforms A, B, more than 60% of maximum activity | Pseudomonas fluorescens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
30 min, isoform B 59% residual activity, isoform C 55% residual activity | Pseudomonas fluorescens |
| 40 | - |
isoform A, 30 min, 70% residual activity | Pseudomonas fluorescens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Pseudomonas fluorescens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 11 | activity range, profile, overview | Pseudomonas fluorescens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7 | alginate lyase C, stable | Pseudomonas fluorescens |
| 5 | 7 | isoform C | Pseudomonas fluorescens |
| 5 | 9 | alginate lyase A and B, stable | Pseudomonas fluorescens |
| 5 | 9 | isoforms A and B | Pseudomonas fluorescens |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Pseudomonas fluorescens | alginate lyase A, isoelectri focusing | - |
4 |
| Pseudomonas fluorescens | isoform A, isoelectric focusing | - |
4 |
| Pseudomonas fluorescens | alginate lyase B, isoelectri focusing | - |
4.36 |
| Pseudomonas fluorescens | isoform B, isoelectric focusing | - |
4.4 |
| Pseudomonas fluorescens | alginate lyase C, isoelectri focusing | - |
4.59 |
| Pseudomonas fluorescens | isoform C, isoelectric focusing | - |
4.6 |
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