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Literature summary for 4.2.2.3 extracted from
- Protoplast preparation from Laminaria japonica with recombinant alginate lyase and cellulase (2011), Mar. Biotechnol., 13, 256-263.
Application
| Application | Comment | Organism |
|---|---|---|
| additional information | use of recombinant abalone alginate lyase and beta-1,4-endoglucanase for protoplast isolation from Laminaria japonica. In Laminaria japonica blades pretreated with proteinase K and incubated in artificial seawater containing alginate lyase and beta-1,4-endoglucanase, the protoplast number is increased up to 5000000 protoplasts/g fresh weight. These cells are mostly derived from the epidermal layer rather than the cortical layer. Results suggest that at least three enzymes, alginate lyase, cellulase, and protease, are essential for effective protoplast isolation from Laminaria japonica | Haliotis discus hannai |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| baculoviral expression system | Haliotis discus hannai |
| expressed as secreted proteins with baculoviral expression systems in Sf9 cells | Haliotis discus hannai |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Haliotis discus hannai | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation and Ni-NTA column chromatography | Haliotis discus hannai |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the specific activity of the recombinant enzyme at 30°C in buffer containing 10 mM NaPi (pH 8.0) is 2490 units/mg, one unit of lyase activity is defined as the amount of enzyme that increases the absorbance at 235 nm by 0.01 unit after 1 min | Haliotis discus hannai |
| 2490 | - |
30°C, pH 8.0 | Haliotis discus hannai |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alginate | recombinant Aly has the capacity to degrade alginate components for protoplast isolation | Haliotis discus hannai | unsaturated algino-oligosaccharides | - |
? |  |
| alpha-L-mannuronyl linkage in alginate | - |
Haliotis discus hannai | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alginate lyase | - |
Haliotis discus hannai |
| ALY | - |
Haliotis discus hannai |
| oligoalginate lyase | - |
Haliotis discus hannai |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Haliotis discus hannai |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 45 | - |
Haliotis discus hannai |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
30 min, 50% residual activity | Haliotis discus hannai |
| 50 | - |
the activity of recombinant Aly is decreased by 50% after incubation at 50°C for 30 min | Haliotis discus hannai |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.7 | - |
- |
Haliotis discus hannai |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9.5 | - |
Haliotis discus hannai |
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Release 2023.1 (January 2023)
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