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Literature summary for 4.2.2.3 extracted from
- Characterization of three new Azotobacter vinelandii alginate lyases, one of which is involved in cyst germination (2009), J. Bacteriol., 191, 4845-4853.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli DH5alpha cells | Azotobacter vinelandii |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | the activity of isoform AlyA3 is stimulated by Ca2+, an increase by 5fold is observed upon increasing the concentration of Ca2+ from 0.02 mM to 6.3 mM | Azotobacter vinelandii |  |
| additional information | the activities of isoforms AlyA1 and AlyA2 are not affected by addition of similar concentrations of Ca2+ | Azotobacter vinelandii |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25900 | - |
isoform AlyA1, calculated from amino acid sequence | Azotobacter vinelandii |
| 26600 | - |
isoform AlyA2, calculated from amino acid sequence | Azotobacter vinelandii |
| 49400 | - |
isoform AlyA3, calculated from amino acid sequence | Azotobacter vinelandii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Azotobacter vinelandii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| HiTrap SP column chromatography (isoform AlyA1), HiTrap Q column chromatography (isoform AlyA2 and AlyA3), and butyl Sepharose column chromatography (isoform AlyA2) | Azotobacter vinelandii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alginate | isoforms AlyA1, AlyA2, and AlyA3 preferably cleave the bond between guluronic acid and mannuronic acid, resulting in a guluronic acid residue at the new reducing end, but isoform AlyA3 also degrades the other three possible bonds in alginate | Azotobacter vinelandii | unsaturated algino-oligosaccharides | - |
? | |
| G-alginate | purified alpha-L-guluronic acid blocks containing more than 90% alpha-L-guluronic acid | Azotobacter vinelandii | ? | - |
? | |
| M-alginate | pure 1-4-linked beta-D-mannuronic acid | Azotobacter vinelandii | ? | - |
? | |
| MG-alginate | alginate epimerized by AlgE4 and containing 47% alpha-L-guluronic acid with no alpha-L-guluronic acid blocks, 100% activity | Azotobacter vinelandii | ? | isoforms AlyA1, AlyA2, and AlyA3 produce mainly DELTAG and DELTAGMG as their end products, isoform AlyA3 produces as many trimers as it does dimers and tetramers | ? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alginate lyase | - |
Azotobacter vinelandii |
| AlyA1 | isoform | Azotobacter vinelandii |
| AlyA2 | isoform | Azotobacter vinelandii |
| AlyA3 | isoform | Azotobacter vinelandii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
isoform AlyA2 | Azotobacter vinelandii |
| 7.5 | - |
isoform AlyA3 | Azotobacter vinelandii |
| 7.8 | - |
isoform AlyA1 | Azotobacter vinelandii |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Azotobacter vinelandii | isoform AlyA3, calculated from amino acid sequence | - |
4.2 |
| Azotobacter vinelandii | isoform AlyA2, calculated from amino acid sequence | - |
5.6 |
| Azotobacter vinelandii | isoform AlyA1, calculated from amino acid sequence | - |
9.7 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | strains lacking alyA3 germinate poorly compared to wild type cells | Azotobacter vinelandii |
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