Activating Compound | Comment | Organism | Structure |
---|---|---|---|
(NH4)2SO4 | 409% relative activity at 200 mM | Agarivorans sp. | |
CH3COO- NH4+ | 298% relative activity at 200 mM | Agarivorans sp. | |
CH3COONa | 233% relative activity at 200 mM | Agarivorans sp. | |
K2HPO4 | 439% relative activity at 200 mM | Agarivorans sp. | |
KCl | 521% relative activity at 200 mM | Agarivorans sp. | |
NaBr | 712% relative activity at 200 mM | Agarivorans sp. | |
NaCl | best activator, 823% relative activity at 200 mM, the activity is increased to 1.8times by adding 0.2 M NaCl, the activity reaches maximum at 0.6-0.8 M NaCl, which is around 20 times without NaCl | Agarivorans sp. | |
NaF | 419% relative activity at 200 mM | Agarivorans sp. | |
NH4Cl | 549% relative activity at 200 mM | Agarivorans sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 95% inhibition at 1 mM | Agarivorans sp. | |
CuSO4 | 7% inhibition at 1 mM | Agarivorans sp. | |
EDTA | 86%inhibition at 5 mM | Agarivorans sp. | |
EGTA | 86% inhibition at 5 mM | Agarivorans sp. | |
Hg2+ | 80% inhibition at 1 mM | Agarivorans sp. | |
Mn2+ | 28% inhibition at 1 mM | Agarivorans sp. | |
additional information | Ni2+, Co2+, and Mg2+ ions do not affect enzyme activity, dithiothreitol and 2-mercaptoethanol (5 mM each) do not affect the enzyme activity at all | Agarivorans sp. | |
Ni2+ | 95% inhibition at 1 mM | Agarivorans sp. | |
Pd2+ | 95% inhibition at 1 mM | Agarivorans sp. | |
Sr2+ | 46% inhibition at 1 mM | Agarivorans sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | 111% relative activity at 1 mM | Agarivorans sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
SDS-PAGE | Agarivorans sp. |
32300 | - |
calculated from amino acid sequence | Agarivorans sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agarivorans sp. | B5BSV2 | - |
- |
Agarivorans sp. JAM-A1m | B5BSV2 | - |
- |
Purification (Comment) | Organism |
---|---|
SuperQ Toyopearl column chromatography, butyl Toyopearl column chromatography, and ammonium sulfate fractionation | Agarivorans sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.142 | - |
crude extract, at pH 10.0 | Agarivorans sp. |
108.5 | - |
after 764.1fold purification, at pH 10.0 | Agarivorans sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alginate | the enzyme degrades alginate by endo fashion, the enzyme degrades favorably mannuronate-guluronate and guluronate-rich fragments in alginate, shows substrate specificity toward beta-D-mannuronic acid blocks of hydrolysed alginate | Agarivorans sp. | unsaturated algino-oligosaccharides | 4-deoxy-L-erythro-hex-4-enopyranosyluronic acids as the non-reducing terminal moiety are contained in the products | ? | |
alginate | the enzyme degrades alginate by endo fashion, the enzyme degrades favorably mannuronate-guluronate and guluronate-rich fragments in alginate, shows substrate specificity toward beta-D-mannuronic acid blocks of hydrolysed alginate | Agarivorans sp. JAM-A1m | unsaturated algino-oligosaccharides | 4-deoxy-L-erythro-hex-4-enopyranosyluronic acids as the non-reducing terminal moiety are contained in the products | ? |
Synonyms | Comment | Organism |
---|---|---|
A1m | - |
Agarivorans sp. |
alginate lyase | - |
Agarivorans sp. |
polymannuronate lyase | - |
Agarivorans sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
around 30°C | Agarivorans sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | A1m is rapidly inactivated upon incubation at 30-50°C for 15 min in 20 mM glycine-NaOH buffer (pH 9) with or without 0.2 M NaCl. The residual activity after treatment at 30°C with 0.2 M NaCl is 53% of the residual activity without NaCl. The residual activities after treatment at 50°C are 10% of the initial activities after incubation at 30°C with or without NaCl. Activation of the enzyme by NaCl is prominent between 25 and 35°C | Agarivorans sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
the optimal pH is around 10 in glycine-NaOH buffer | Agarivorans sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | A1m is quite stable in incubation at 30°C for 1 h between pH 6.0 and 9.0. When the optimal pH in the presence of 0.2 M NaCl is shifted to pH 9.0, the activity is more than 10times compared with that at pH 9 in the absence of NaCl. Similar results are observed in 100 mM borate buffer with or without 0.2 M NaCl. The stability of A1m in various pH buffers is unaffected by adding NaCl | Agarivorans sp. |