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Literature summary for 4.2.2.3 extracted from

  • Duan, G.; Han, F.; Yu, W.
    Cloning, sequence analysis, and expression of gene alyPI encoding an alginate lyase from marine bacterium Pseudoalteromonas sp. CY24 (2009), Can. J. Microbiol., 55, 1113-1118.
    View publication on PubMed
Search for more literature for 4.2.2.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Pseudoalteromonas sp.

Inhibitors

Inhibitors Comment Organism Structure
(NH4)2SO4 96% residual activity at 1 mM Pseudoalteromonas sp.
Ba2+ 79% residual activity at 1 mM Pseudoalteromonas sp.
EDTA 56% residual activity at 1 mM Pseudoalteromonas sp.
K+ 67% residual activity at 100 mM Pseudoalteromonas sp.
Mg2+ 87% residual activity at 1 mM Pseudoalteromonas sp.
Na+ 59% residual activity at 900 mM Pseudoalteromonas sp.
Ni2+ 67% residual activity at 1 mM Pseudoalteromonas sp.
SDS 35% residual activity at 1 mM Pseudoalteromonas sp.
Zn2+ 68% residual activity at 1 mM Pseudoalteromonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.36
-
 alginate in 50 mM phosphate buffer, pH 7.0, at 40°C Pseudoalteromonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 136% relative activity at 1 mM Pseudoalteromonas sp.
Fe3+ 147% relative activity at 1 mM Pseudoalteromonas sp.
K+ 117% relative activity at 50 mM Pseudoalteromonas sp.
Mn2+ 150% relative activity at 1 mM Pseudoalteromonas sp.
Na+ 280% relative activity at 100 mM Pseudoalteromonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57400
-
 calculated from amino acid sequence Pseudoalteromonas sp.
58000
-
 SDS-PAGE Pseudoalteromonas sp.

Organism

Organism UniProt Comment Textmining
Pseudoalteromonas sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-Sepharose column chromatography Pseudoalteromonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alginate
-
 Pseudoalteromonas sp. unsaturated algino-oligosaccharides
-
 ?

Synonyms

Synonyms Comment Organism
alginate lyase
-
 Pseudoalteromonas sp.
AlyPI
-
 Pseudoalteromonas sp.
polymannuronate lyase
-
 Pseudoalteromonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 in the presence of 0.1 M NaCl Pseudoalteromonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 50 the residual activity decreases when the temperature goes above 20°C and disappears at 50°C Pseudoalteromonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 in the presence of 0.1 M NaCl Pseudoalteromonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
6 10.6
-
 Pseudoalteromonas sp.