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Literature summary for 4.2.2.3 extracted from
- Overexpression of alginate lyase of Pseudoalteromonas elyakovii in Escherichia coli, purification, and characterization of the recombinant alginate lyase (2008), World J. Microbiol. Biotechnol., 24, 89-96.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| BaCl2 | - |
Pseudoalteromonas elyakovii |  |
| CaCl2 | - |
Pseudoalteromonas elyakovii | |
| KCl | - |
Pseudoalteromonas elyakovii | |
| MgCl2 | - |
Pseudoalteromonas elyakovii | |
| MnCl2 | - |
Pseudoalteromonas elyakovii | |
| NaCl | - |
Pseudoalteromonas elyakovii | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpressed in Escherichia coli | Pseudoalteromonas elyakovii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | relative activity: 95% | Pseudoalteromonas elyakovii | |
| AgNO3 | relative activity: 28% | Pseudoalteromonas elyakovii | |
| CoCl2 | relative activity: 717 | Pseudoalteromonas elyakovii | |
| EDTA | relative activity: 48% | Pseudoalteromonas elyakovii | |
| EGTA | relative activity: 71% | Pseudoalteromonas elyakovii | |
| PMSF | relative activity: 95% | Pseudoalteromonas elyakovii | |
| SDS | - |
Pseudoalteromonas elyakovii | |
| ZnSO4 | relative activity: 78% | Pseudoalteromonas elyakovii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| BaCl2 | stimulates activity, relative activity: 198% | Pseudoalteromonas elyakovii | |
| CaCl2 | stimulates activity, relative activity: 176% | Pseudoalteromonas elyakovii | |
| KCl | stimulates activity, relative activity: 109% | Pseudoalteromonas elyakovii | |
| MgCl2 | stimulates activity, relative activity: 156% | Pseudoalteromonas elyakovii | |
| MnCl2 | stimulates activity, relative activity: 121% | Pseudoalteromonas elyakovii | |
| NaCl | stimulates activity, relative activity: 113% | Pseudoalteromonas elyakovii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
SDS-PAGE | Pseudoalteromonas elyakovii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudoalteromonas elyakovii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using gel filtraton and anion-exchange chromatography | Pseudoalteromonas elyakovii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alginate | - |
Pseudoalteromonas elyakovii | unsaturated algino-oligosaccharides | - |
? | |
| poly alpha-L-guluronate | - |
Pseudoalteromonas elyakovii | oligo-alpha-1,4-L-guluronate | - |
? | |
| poly beta-D-mannuronate | - |
Pseudoalteromonas elyakovii | oligo-beta-1,4-D-mannuronate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alginate lyase | - |
Pseudoalteromonas elyakovii |
| alyPEEC | - |
Pseudoalteromonas elyakovii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Pseudoalteromonas elyakovii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
enzyme totally inactivated at 70°C within 10 min | Pseudoalteromonas elyakovii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Pseudoalteromonas elyakovii |
| 7.5 | - |
assay at | Pseudoalteromonas elyakovii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7 | - |
Pseudoalteromonas elyakovii |
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