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Literature summary for 4.2.2.3 extracted from

  • Cao, L.; Xie, L.; Xue, X.; Tan, H.; Liu, Y.; Zhou, S.
    Purification and characterization of alginate lyase from Streptomyces species strain A5 isolated from banana rhizosphere (2007), J. Agric. Food Chem., 55, 5113-5117.
    View publication on PubMed
Search for more literature for 4.2.2.3

Activating Compound

Activating Compound Comment Organism Structure
EDTA enhances activity Streptomyces sp.

Application

Application Comment Organism
agriculture the alginate oligomers prepared by the lyase from Streptomyces sp. A5 show growth-promoting activity on the roots of banana plantlets. Encapsulation method using alginate microbeads to inoculate beneficial streptomycete strains might be beneficial to the root growth of banana plantlets Streptomyces sp.

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 2 mM Streptomyces sp.
Fe3+ 2 mM Streptomyces sp.
Hg2+ 2 mM Streptomyces sp.
SDS 0.05 M Streptomyces sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km for sodium alginate is 0.13 mg/ml Streptomyces sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Streptomyces sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 x * 32000, SDS-PAGE Streptomyces sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alginate Streptomyces sp. the alginate oligomers prepared by the lyase show growth-promoting activity on the roots of banana plantlets (Streptomyces sp. A5 is isolated from banana rhizosphere) ?
-
 ?
alginate Streptomyces sp. A5 the alginate oligomers prepared by the lyase show growth-promoting activity on the roots of banana plantlets (Streptomyces sp. A5 is isolated from banana rhizosphere) ?
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces sp.
-
 isolated from banana rhizosphere
-
Streptomyces sp. A5
-
 isolated from banana rhizosphere
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alginate the alginate oligomers prepared by the lyase show growth-promoting activity on the roots of banana plantlets (Streptomyces sp. A5 is isolated from banana rhizosphere) Streptomyces sp. ?
-
 ?
alginate substrate specificity for polyguluronate units in the alginate molecules Streptomyces sp. ?
-
 ?
alginate the alginate oligomers prepared by the lyase show growth-promoting activity on the roots of banana plantlets (Streptomyces sp. A5 is isolated from banana rhizosphere) Streptomyces sp. A5 ?
-
 ?
alginate substrate specificity for polyguluronate units in the alginate molecules Streptomyces sp. A5 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 32000, SDS-PAGE Streptomyces sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Streptomyces sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 92% of the activity is lost after incubation at 70°C and pH 7.5 for 20 min Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Streptomyces sp.