Literature summary for 4.2.2.3 extracted from

Shen, H.; Han, F.; Lin, Y.; Yu, W.
A high efficient electroporation of Pseudomonas sp. QDA pretreated with alginate lyase (2006), Enzyme Microb. Technol., 39, 677-682.

No PubMed abstract available

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Application

Application	Comment	Organism
biotechnology	most of Pseudomonas strains have copious alginates enclosing the bacteria cells, which makes it difficult to transfer exogenous DNA into the cells during transformation. Pretreatment of Pseudomonas sp. QDA with alginate lyase before electroporation increases transformation efficiency approximately 10000fold than without pretreatment of alginate lyase, and a high transformation efficiency nearly the same as that of alginate production deficient mutant is obtained. Among the alginate lyases tested, AL2 is the most effective enzyme for pretreatment. This electroporation procedure is also efficient for Pseudomonas aeruginosa FRD1 (mucoid) and PAO1 (non-mucoid)	Vibrio sp.

Organism

Organism	UniProt	Comment	Textmining
Vibrio sp.	-	-	-

Synonyms

Synonyms	Comment	Organism
AL2	-	Vibrio sp.

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Release 2023.1 (January 2023)