Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Sphingomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 1 mM, 50% inhibition | Sphingomonas sp. | |
Cu2+ | 1 mM, more than 95% inhibition | Sphingomonas sp. | |
Hg2+ | 1 mM, more than 95% inhibition | Sphingomonas sp. | |
Zn2+ | 1 mM, more than 95% inhibition | Sphingomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Sphingomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
85000 | - |
gel filtration | Sphingomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Sphingomonas sp. | A1-1V' has no significant role in alginate metabolism | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingomonas sp. | Q5DWP2 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant | Sphingomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
16.1 | - |
- |
Sphingomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alginate | - |
Sphingomonas sp. | unsaturated alginate disaccharides + unsaturated alginate trisaccharides | main products | ? | |
additional information | A1-1V' has no significant role in alginate metabolism | Sphingomonas sp. | ? | - |
? | |
poly-alpha-L-guluronate | 14.9% of the activity with poly-beta-D-mannuronate | Sphingomonas sp. | ? | - |
? | |
poly-beta-D-mannuronate | - |
Sphingomonas sp. | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
A1-IV' | - |
Sphingomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Sphingomonas sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 65 | 30°C: about 50% of maximal activity, 65°C: about 55% of maximal activity | Sphingomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
10 min, 85% loss of activity | Sphingomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.5 | - |
Sphingomonas sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | pH 6.0: about 55% of maximal activity, pH 10.0: about 60% of maximal activity | Sphingomonas sp. |