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Literature summary for 4.2.2.3 extracted from

  • Hashimoto, W.; Miyake, O.; Ochiai, A.; Murata, K.
    Molecular identification of Sphingomonas sp. A1 alginate lyase (A1-IV') as a member of novel polysaccharide lyase family 15 and implications in alginate lyase evolution (2005), J. Biosci. Bioeng., 99, 48-54.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Sphingomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 1 mM, 50% inhibition Sphingomonas sp.
Cu2+ 1 mM, more than 95% inhibition Sphingomonas sp.
Hg2+ 1 mM, more than 95% inhibition Sphingomonas sp.
Zn2+ 1 mM, more than 95% inhibition Sphingomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Sphingomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
85000
-
gel filtration Sphingomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Sphingomonas sp. A1-1V' has no significant role in alginate metabolism ?
-
?

Organism

Organism UniProt Comment Textmining
Sphingomonas sp. Q5DWP2
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Sphingomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
16.1
-
-
Sphingomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alginate
-
Sphingomonas sp. unsaturated alginate disaccharides + unsaturated alginate trisaccharides main products ?
additional information A1-1V' has no significant role in alginate metabolism Sphingomonas sp. ?
-
?
poly-alpha-L-guluronate 14.9% of the activity with poly-beta-D-mannuronate Sphingomonas sp. ?
-
?
poly-beta-D-mannuronate
-
Sphingomonas sp. ?
-
?

Synonyms

Synonyms Comment Organism
A1-IV'
-
Sphingomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Sphingomonas sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 65 30°C: about 50% of maximal activity, 65°C: about 55% of maximal activity Sphingomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
10 min, 85% loss of activity Sphingomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5
-
Sphingomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
6 10 pH 6.0: about 55% of maximal activity, pH 10.0: about 60% of maximal activity Sphingomonas sp.