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Literature summary for 4.2.2.3 extracted from

  • Yamasaki, M.; Moriwaki, S.; Miyake, O.; Hashimoto, W.; Murata, K.; Mikami, B.
    Structure and function of a hypothetical Pseudomonas aeruginosa protein PA1167 classified into family PL-7: a novel alginate lyase with a beta-sandwich fold (2004), J. Biol. Chem., 279, 31863-31872.
    View publication on PubMed
Search for more literature for 4.2.2.3

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of AlgL in Escherichia coli Pseudomonas aeruginosa
protein PA1167, overexpression in Escherichia coli Pseudomonas aeruginosa

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop vapour diffusion method, crystal structure determined at 2.0 A resolution. PA1167 forms a glove-like beta-sandwich composed of 15 beta-strands and 3 alpha-helices Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
 protein PA1167, gel filtration Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alginate Pseudomonas aeruginosa AlgL plays a main role in alginate depolymerization unsaturated algino-oligosaccharides
-
 ?
alginate Pseudomonas aeruginosa AlgL AlgL plays a main role in alginate depolymerization unsaturated algino-oligosaccharides
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
 AlgL
-
Pseudomonas aeruginosa
-
 protein PA1167
-
Pseudomonas aeruginosa AlgL
-
 AlgL
-

Purification (Commentary)

Purification (Comment) Organism
recombinant AlgL Pseudomonas aeruginosa
recombinant protein PA1167 Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0274
-
 protein PA1167 Pseudomonas aeruginosa
0.926
-
 AlgL Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alginate
-
 Pseudomonas aeruginosa unsaturated algino-oligosaccharides
-
 ?
alginate AlgL plays a main role in alginate depolymerization Pseudomonas aeruginosa unsaturated algino-oligosaccharides
-
 ?
alginate protein PA1167 acts preferably on heteropolymeric regions endolytically Pseudomonas aeruginosa unsaturated algino-oligosaccharides
-
 ?
alginate
-
 Pseudomonas aeruginosa AlgL unsaturated algino-oligosaccharides
-
 ?
alginate AlgL plays a main role in alginate depolymerization Pseudomonas aeruginosa AlgL unsaturated algino-oligosaccharides
-
 ?
alginate protein PA1167 acts preferably on heteropolymeric regions endolytically Pseudomonas aeruginosa AlgL unsaturated algino-oligosaccharides
-
 ?

Subunits

Subunits Comment Organism
? x * 25000, protein PA1167, SDS-PAGE Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
AlgL
-
 Pseudomonas aeruginosa
protein PA1167
-
 Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 protein PA1167 Pseudomonas aeruginosa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 10 min, 80% loss of activity, protein PA1167 Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.42
-
 alginate protein PA1167, pH 7.5, 37°C Pseudomonas aeruginosa
748
-
 alginate AlgL Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 protein PA1167 Pseudomonas aeruginosa