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Literature summary for 4.2.2.3 extracted from
- Cloning, sequence analysis and expression of gene alyVI encoding alginate lyase from marine bacterium Vibrio sp. QY101 (2004), DNA Seq., 15, 344-350.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Vibrio sp. |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| SDS | 1 mM, 94% loss of activity | Vibrio sp. |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.09 | - |
alginate | - |
Vibrio sp. | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | 1 mM, enhances activity by 66% | Vibrio sp. | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vibrio sp. | Q53EJ1 | - |
- |
| Vibrio sp. QY101 | Q53EJ1 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | signal peptide is cleaved off leaving a mature protein of 34000 Da | Vibrio sp. |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant | Vibrio sp. |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture supernatant | - |
Vibrio sp. | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alginate | - |
Vibrio sp. | algino-monosaccharides + algino-disaccharides | - |
? | |
| alginate | - |
Vibrio sp. QY101 | algino-monosaccharides + algino-disaccharides | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AlyVI | - |
Vibrio sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Vibrio sp. |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 45 | 30°C: about 60% of maximal activity, 45°C: about 70% of maximal activity | Vibrio sp. |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 60 | 1 h, residual activity decreases when temperature goes above 30°C and disappears at 60°C | Vibrio sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Vibrio sp. |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8.5 | pH 5.0: about 40% of maximal activity, pH 8.5: about 35% of maximal activity | Vibrio sp. |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10.5 | 30°C, 30 min | Vibrio sp. |
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