Literature summary for 4.2.2.3 extracted from

Yoon, H.J.; Hashimoto, W.; Miyake, O.; Murata, K.; Mikami, B.
Crystal structure of alginate lyase A1-III complexed with trisaccharide product at 2.0 A resolution (2001), J. Mol. Biol., 307, 9-16.

Search for more literature for 4.2.2.3

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression of isozyme A1-III in Bacillus subtilis	Sphingomonas sp.

Crystallization (Commentary)

Crystallization (Comment)	Organism
8 mg/ml purified isozyme A1-III complexed with trisaccharide product 4-deoxy-L-erythro-hex-4-enepyranosyluronate-mannuronate-mannuronic acid, hanging drop vapour diffusion method, 0.1 M HEPES, pH 7.5, containg 48% PEG w/v saturated ammonium sulfate, 290 mM trisaccharide, 20°C, X-ray diffraction structure determination and analysis at 2.0 A resolution	Sphingomonas sp.

Organism

Organism	UniProt	Comment	Textmining
Sphingomonas sp.	-	isozyme A1-III	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant isozyme A1-III from Bacillus subtilis	Sphingomonas sp.

Reaction

Reaction	Comment	Organism	Reaction ID
R2-beta-D-mannuronic acid-R1 = R2-OH + 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-R1	substrate binding site structure, active site cleft, catalytic mechanism	Sphingomonas sp.	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
poly-beta-1,4-D-mannuronate	-	Sphingomonas sp.	4-deoxy-L-erythro-hex-4-enepyranosyluronate-mannuronate-mannuronic acid	-	?

Synonyms

Synonyms	Comment	Organism
alginate lyase	-	Sphingomonas sp.

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Release 2023.1 (January 2023)