Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion method, 0.003 ml protein solution: 17.2 mg/ml protein, 0.1 M HEPES, pH 7.5, + 0.003 ml bottom solution: 47-49% saturated ammonium sulfate, 0.1 M HEPES, pH 7.5, 20°C, 1 month, for large crystals micro- and macroseeding is used, crystal are soaked in heavy-atom derivatives for 1-4 h for X-ray diffraction structure determination and analysis at 1.78-10.0 A resolution | Sphingomonas sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
sulfate | sulfate binding site structure | Sphingomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingomonas sp. | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
poly-beta-D-mannuronate | i.e. alginate, cleavage site structure | Sphingomonas sp. | oligomannuronate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme shows an (alpha6/alpha5)-barrel structure with a tunnel-like active site cleft | Sphingomonas sp. |
Synonyms | Comment | Organism |
---|---|---|
alginate lyase1-III | - |
Sphingomonas sp. |
ALYIII | - |
Sphingomonas sp. |